Dynamic changes of microbial community diversity in a photohydrogen producing reactor monitored by PCR-DGGE  

作　　者：YING Yanling LV Zhenmei MIN Hang CHENG Jun 

机构地区：[1]Institution of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China [2]State Key Lab of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China

出　　处：《Journal of Environmental Sciences》2008年第9期1118-1125,共8页环境科学学报（英文版）

基　　金：the National NaturalScience Foundation (No. 30570053);the Fund of CrossSubjects of Zhejiang University (No. 109000-812651);the National Natural Science Foundation of China (No.50406022);the National High Technology Research and Development Program of China (No. 2007AA10Z409);the National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science &Technology of China (No. 2006BAJ08B01)

摘　　要：A PCR-DGGE (denaturing gradient gel electrophoresis of polymerase chain reaction) protocol was used for monitoring the dynamic changes in the microbial population during photohydrogen production. Total DNA was extracted directly from the mixed bacterial community in the reactor and subjected to PCR with V3-16S rDNA and pufM gene primers, and the amplifications were then analyzed by DGGE. The DGGE patterns demonstrated the dynamics of community structure and the shift of microbial diversity, which correspond...A PCR-DGGE (denaturing gradient gel electrophoresis of polymerase chain reaction) protocol was used for monitoring the dynamic changes in the microbial population during photohydrogen production. Total DNA was extracted directly from the mixed bacterial community in the reactor and subjected to PCR with V3-16S rDNA and pufM gene primers, and the amplifications were then analyzed by DGGE. The DGGE patterns demonstrated the dynamics of community structure and the shift of microbial diversity, which correspond...

关 键 词：PCR-DGGE V3-16S rDNA region pufM gene photohydrogen producing reactor 

分 类 号：X172[环境科学与工程—环境科学]