电穿孔诱发细胞凋亡和提高抗癌药物细胞毒性的研究  

Induction of Apoptosis by Electroporation and Study of Higher Cytotoxity of Anticancer Drugs Due to Electroporation

作　　者：孙敬儒[1] 王子淑[1] 刘一勇[1] 邹方东[1] 钱宇[2] 王喜忠[1] 陈文元[1] 郭聪[1] 王保义[3] 张弘[3] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]四川省食品发酵研究院,成都611130 [3]四川大学无线电系,成都610064

出　　处：《四川大学学报（自然科学版）》2002年第S1期170-174,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金 (6 0 0 710 2 4)

摘　　要：作者报道了电穿孔可以诱发HeLa细胞凋亡 ,采用Giemsa染料、吖啶橙染色及Hoechst33342 ,PI双染的荧光染色 ,研究了电穿孔对HeLa细胞凋亡的影响 .以HeLa细胞和胎儿脐带血淋巴细胞为材料 ,进行了电穿孔提高抗癌药物细胞毒性的研究 ,为电化学疗法在体内实验的可行性提供了依据 .That electroporation can induce apoptosis is investigated in HeLa cells, the effects of electroporation to apoptosis of HeLa cells is studied with Giemsa,Acridine orange,Hoechst33342 and propidium iodide staining.HeLa cells and fetal navel cord blood lymphocytes are chosen as research objects in the experiement,which determines whethere or not electroporation can enchance cytotoxity of anticancer drugs,The results can testify the feasibility of electrochemo therapy(ECT) trials in vivo.

关 键 词：电穿孔 细胞凋亡 抗癌药物 电化学疗法 

分 类 号：R73-36[医药卫生—肿瘤]