烟草叶细胞植株再生的研究  

Study on Plantlet Regeneration from Leaf Cells of Tobacco

作　　者：王煜[1] 杨雨晗[1] 罗英[2] 陈今朝[3] 韩善华[1] 

机构地区：[1]四川师范大学细胞生物学研究室,成都610068 [2]四川绵阳师范高等专科学校生物系,四川绵阳621000 [3]重庆涪陵师范学院生物系,重庆400000

出　　处：《四川大学学报（自然科学版）》2002年第S1期4-7,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省教育厅基金;重庆市教育厅重点项目基金

摘　　要：在一定的培养条件下 ,将无菌苗叶块接种在含有 0 .5mg/LNAA的MS基本培养基上 ,可诱导出愈伤组织 ;在 0 .0 5mg/LNAA + 1.5mg/L 6 BA的MS培养基上能分化出芽 ;在 0 .1mg/LIAA的MS培养基中能形成根 .作者在文中还讨论了烟草叶细胞脱分化和再分化与植物激素、蔗糖和培养材料的关系 .The leaf pieces of aseptic shoots were inoculated on MS medium contained 0.5mg/L NAA in the conditions which temperature was 25℃, the intensity of illumination was 5000lx and 10h photoperiod, a lot of calli was induced from leaf pieces. Buds could appear on the faces of calli on MS medium contained 0.05mg/L NAA and 1.5mg/L 6-BA. Roots should form in MS medium contained 0.1mg/L IAA. The authors still discussed the relation of dedifferentiation and redifferentiation of leaf cells of tobacco with plant hormon...

关 键 词：烟草 叶细胞 愈伤组织 再生植株 

分 类 号：S572[农业科学—烟草工业]