多重人肝癌多药耐药细胞模型的建立  

Establishment of Complex Multidrug Resistance Cell Sublines of Human Liver Cancer

作　　者：洪伟[1] 林永堃[1] 石铮[1] 刘景丰[1] 

机构地区：[1]福建医科大学附属第一医院普外科,福州350005

出　　处：《福建医科大学学报》2001年第S1期39-42,共4页Journal of Fujian Medical University

摘　　要：目的　研究肝癌多药耐药 (MDR)机制。　方法　采用阿霉素 +丝裂霉素通过药物间断 /持续接触诱导法诱导 SMMC 772 1细胞株建立一组多重人肝癌 MDR细胞模型 SMMC 772 1/ M细胞亚系。　结果　多重人肝癌MDR亚系 SMMC 772 1/ M的耐药涉及 mdr1 、MRP、L RP、Topo α和 GSTP1 基因。　结论　 SMMC 772 1/ M亚株是目前肝癌Objective\ To study multidrug resistance(MDR) mechanism of hepatocellular carcinoma(HCC).\ Methods\ By using ADM+MMC of chemotheraputic agents HCC cell lines\|SMMC 7721, a group of complex MDR cell sublines were successfully established.\ Results\ Observation on the model's drug resistance mechanism revealed that drug resistance in the complex\|MDR subline SMMC 7721/M was mediated by mdr\-1, MRP, LRP, TopoⅡα and GST\-\{P1\} genes collectively.\ Conclusion\ The established SMMC 7721/M subline was an optimal ...

关 键 词：癌 肝细胞 抗药性 多药 细胞耐受性 基因表达 

分 类 号：R735.7[医药卫生—肿瘤]