口腔扁平苔藓癌变潜能探讨——P53基因表达及形态计量学研究  

Thy study of malignant petential:P53 expression and cell morphometric analysis in oral lichen planus

作　　者：蔡扬[1] 于燕妮[2] 肖开棋[2] 

机构地区：[1]贵阳医学院附院口腔科,贵州贵阳550004 [2]贵阳医学院附院病理科,贵州贵阳550004

出　　处：《临床口腔医学杂志》2001年第z1期7-9,共3页Journal of Clinical Stomatology

摘　　要：目的　从P5 3基因的表达及细胞形态计量变化 ,研究口腔扁平苔藓 (OLP)癌变的潜能。方法　采用免疫组化SABC法对 2 8例OLP病损P5 3基因表达情况进行检测 ;用图像分析技术 ,对其中 16例OLP的细胞形态学变化进行测量及分析 ,并分别与正常口腔粘膜、上皮异常增生性白斑、及鳞癌比较。结果　P5 3蛋白在正常口腔粘膜中表达阴性 ,但可在OLP、白斑 (LK)角朊细胞及鳞癌 (SCC)癌细胞中表达 ,阳性细胞数比例由OLP、LK到SCC依次递增 ,差异有显著性 (P <0 0 5 )。细胞形态定量结果显示 :OLP各项参数值介于正常与白斑和鳞癌之间 ,与鳞癌差异显著 (P <0 0 5 )。结论　OLP是一种界于正常及癌之间的良性病损 ,具有一定的恶变潜能 。Objective It was reported that a few cases of oral lichen planus (OLP) eventually developed into squamous cell carcinomas (SCC) and that mutanted P53 correlated closely with the malignant development of hyperplastic lesion of the oropharyngeal mucosa such as leukoplakia and OLP. In the present study, the malignant potential of OLP was detected by P53 protein expression and cell morphometry, compared with dysplastic leukoplakia (LK) and SCC. Methods Specimens of 28 cases of OLP were immunostained with monoclonal anti P53 by SABC method. Cell morphometric parameters were measured and analyzed in 16 of OLP specimens, normal buccal mucosa. LK and SCC serving as the controls. Results ① Prominent P53 protein expression was seen in 46.43% of OLP, 54.55% of LK and 60% of SCC biopsies. but negative in normal buccal mucosa. ② Cell morphometric parameters of OLP were compared with those of normal buccal mucosa, LK and SCC. The difference between SCC and OLP group was significant. Conclusion OLP has been found to be a benign lesion, exerting certain malignant potential, perhaps slighter than LK.

关 键 词：口腔扁平苔藓(OLP) P53 形态计量学 

分 类 号：R780.2[医药卫生—口腔医学] R781.5[医药卫生—临床医学]