杠柳苷对人食管癌细胞增殖的抑制作用及与Rb蛋白表达的关系  

Effects of Periplocin on Proliferation of Human Esophageal Carcinoma Cell TE-13 and Its Relation With Rb Expression

作　　者：单保恩[1] 赵连梅[1] 何兰欣[2] 邸红琴[3] 任凤芝[4] 

机构地区：[1]河北医科大学第四医院科研中心 [2]河北省肿瘤研究所,河北石家庄050011 [3]华北制药集团新药研究开发有限责任公司,河北石家庄050015 [4]河北省胸科医院,河北石家庄050012

出　　处：《癌变．畸变．突变》2008年第5期342-345,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(30371753);河北省自然科学基金资助项目(C2004000610)

摘　　要：背景与目的:分析中药香加皮醇提物杠柳苷(CPP)对食管癌细胞株TE-13的生长抑制作用,探讨其诱导细胞凋亡和周期阻滞与Rb蛋白表达的关系。材料与方法:取对数生长期的人食管癌细胞TE-13,接种于96孔培养板,分为实验组、阴性对照组和阳性对照组。实验组加入不同浓度的CPP,阴性对照孔加入完全培养基,阳性对照孔加入化疗药物羟甲基喜树碱,应用MTT法检测CPP对TE-13细胞的抑制作用;光镜下观察TE-13细胞的形态学变化;流式细胞技术检测细胞凋亡率;免疫组化法检测TE-13细胞经药物处理前后去磷酸化Rb蛋白表达的变化。结果:CPP对TE-13细胞增殖具有明显的抑制作用(P<0.01),其药物浓度越大,作用时间越长,抑制效应越强;CPP作用48 h时,对TE-13细胞的半数抑制浓度(IC50)为0.61μg/ml。出现亚二倍体和凋亡峰(Ap);CPP能提高TE-13细胞中Rb蛋白的表达(P<0.01)。结论:CPP能显著抑制TE-13细胞的增殖,促进其凋亡,其作用机制可能与CPP提高Rb蛋白的表达有关。BACKGROUND AND AIM: Effects of periplocin from cortex periplocae(CPP) on proliferation and apoptosis of human esophageal carcinoma cell and its relation with Rb protein expression were investigated.MATERIALS AND METHODS: Esophageal cancer cell line TE-13 was co-incubated with different concentrations of CPP and cell proliferation was measured by MTT method.Cell morphology changes were examined by light microscope.Apoptosis was studied by flow cytometry.Rb expression was analyzed by immunohistochemistry method.RESULTS: CPP could inhibit the proliferation of TE-13 cells significantly in a dose and time dependent manner(P<0.01).After treatment with CPP,TE-13 cells showed morphologic changes of apoptosis.The expression of Rb was enhanced.CONCLUSION: CPP could inhibit the growth of TE-13 cells,the mechanism might be related to enhanced Rb expression.

关 键 词：杠柳苷 食管癌 RB蛋白 香加皮 

分 类 号：R730.45[医药卫生—肿瘤]