肺泡灌洗液中多聚免疫球蛋白受体分泌片段测定的临床意义  

The Protein Levels and Clinical Significance of pIgR/SC in BALF Derived from Lung Cancer Patients

作　　者：李敏[1] 张蕾[2] 肖汀[1] 张莹[1] 韩迺珺[1] 程书钧[1] 王贵齐[2] 高燕宁[1] 

机构地区：[1]中国医学科学院北京协和医学院肿瘤医院肿瘤研究所病因及癌变研究室,北京100021 [2]中国医学科学院北京协和医学院肿瘤医院肿瘤研究所内镜科,北京100021

出　　处：《癌变．畸变．突变》2008年第5期398-401,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家973规划项目(2004CB518705);北京市教育委员会共建项目(JB100230536);高等学校博士学科点专项科研基金(20050023009)

摘　　要：背景与目的:检测肺癌患者支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中多聚免疫球蛋白受体分泌片段(polymeric immunoglobulin receptor/secretory component,pIgR/SC)的蛋白水平,并探讨其临床意义。材料与方法:采用酶联免疫吸附分析法(ELISA)测定52例肺癌患者患侧肺及其中18例患者配对健侧肺BALF中pIgR/SC的蛋白水平。结果:肺癌患者患侧BALF中pIgR/SC蛋白水平显著高于健侧对照组(P=0.009);肺腺癌患者BALF中pIgR/SC蛋白水平显著高于肺鳞癌患者(P=0.014);而在肺鳞癌患者组,手术时发现淋巴结转移的患者BALF中pIgR/SC蛋白水平显著高于手术时未发现淋巴结转移患者(P=0.012)。结论:BALF中pIgR/SC蛋白水平在肺癌患者患侧较健侧显著升高,肺腺癌患者BALF中pIgR/SC蛋白水平显著高于肺鳞癌患者,BALF中pIgR/SC蛋白水平与肺鳞癌淋巴结转移密切相关。BACKGROUND AND AIM: To evaluate the polymeric immunoglobulin receptor/secretory component(pIgR/SC) protein levels in the bronchoalveolar lavage fluid(BALF) samples derived from lung cancer patients,and explore its clinical significance.MATERIALS AND METHODS: BALF samples were obtained from 52 lung cancer patients from the affected side and also the matched healthy side in 18 patients,and were analyzed for the pIgR/SC protein levels by enzyme-linked immunosorbent assay(ELISA).RESULTS: In the 18 cases,the mean pIgR/SC value in BALF of the affected side was significantly greater than that of the matched healthy side(P=0.019).In 52 lung cancer patients,the protein level of pIgR/SC in BALF was significantly higher in adenocarcinoma patients than that in squamous cell carcinomas(P=0.014).The pIgR/SC levels in BALF of patients with squamous cell carcinoma with lymph node invasion were significantly higher than that of patients without(P=0.012).CONCLUSION: The high level of pIgR/SC in airways is likely to indicate host response to tumor carcinogenesis.Furthermore the pIgR/SC level in BALF may reflect the histological difference of lung cancers.In squamous cell carcinoma,pIgR/SC may be involved in lymph node invasion.

关 键 词：pIgRSC 肺癌 肺泡灌洗液 酶联免疫吸附分析 

分 类 号：R730.45[医药卫生—肿瘤]