电刺激小脑顶核对老年痴呆大鼠海马胶质纤维酸蛋白的表达影响  

Effect of fastigial nucleus stimulation on GFAP expression in hippocampus with Alzheimer disease in rats

作　　者：罗晶[1] 王永红[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科重庆市神经病学高校重点实验室,重庆400016

出　　处：《重庆医科大学学报》2008年第z1期52-54,66,共4页Journal of Chongqing Medical University

摘　　要：目的:研究Aβ1-40诱导阿尔茨海默病(Alzheimer’s disease,AD)大鼠空间学习记忆能力及海马区星形胶质细胞(Astro-cyte,AS)胶质纤维酸蛋白(Glial fibrillary acidic protein,GFAP)表达及电刺激小脑顶核(Fastigial nucleus stimulation,FNS)的干预作用。方法:将Aβ1-40微量注射至大鼠右侧海马,制作AD大鼠模型,干预组给予电刺激小脑顶核和电刺激齿状核(Dentate nucle-us stimulation,DNS),28d后进行水迷宫试验。免疫组织化学法检测GFAP免疫阳性细胞表达。结果:FNS干预后AD大鼠空间学习记忆能力明显改善(P<0.05)。与假手术组相比,AD组、DNS组大鼠脑组织海马区可见星形胶质细胞GFAP表达明显增多(P<0.05),FNS组大鼠脑组织海马区星形胶质细胞GFAP表达较AD组下降(P<0.05)。结论:FNS可部分改善Aβ1-40诱导的AD模型大鼠空间学习记忆障碍,其机制可能与降低GFAP的表达和抑制AS活性有关。Objective:Observe the effects of fastigial nucleus stimulation(FNS) on spatial memory and learning ability of the Aβ1-40 induced Alzheimer rat model,by detecting GFAP expression of hippocampus area. Methods:Aβ1-40 was microinjected into the right hippocampus of rats. Cerebella dentate nucleus and fastigial nucleus were stimulated separately before the model was made in interference group. The spatial memory and learning ability of the rat was evaluated by the Morris water maze procedure at the 28th days after injection. The expression of GFAP in astrocyte cell was determined with immunohistochemistry method.Results:The AD rats received FNS showed significant shorter escape latency than the group without FNS(P<0.05).Aβ1-40 could increase the optical density of positive cells of GFAP(P<0.05),FNS could reduce the optical density of positive cells of GFAP(P<0.05),but in dentate nucleus stimulation group no such effect was found. Conclusion:FNS can partly improve the spatial learning and memory dysfunction of AD rat by inhibits the expression of GFAP of hippocampus area and decrease the activity of astrocyte cell.

关 键 词：淀粉样Β蛋白 星彤胶质细胞 胶质纤维酸蛋白 

分 类 号：R741[医药卫生—神经病学与精神病学]