耐药性癫痫患者脑组织中Bcl-2蛋白的表达及临床意义  

The overexpression of the Bcl-2 protein in the brain of paitents with drug-refractory epilepsy

作　　者：曾艳[1] 王学峰[1] 林涛[1] 何梅[1] 李劲梅[2] 袁洁[1] 王亮[1] 关立峰[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆市神经病学重点实验室,重庆400016 [2]四川大学华西医学院附属第一医院神经内科,四川610041

出　　处：《重庆医科大学学报》2008年第z1期70-72,共3页Journal of Chongqing Medical University

摘　　要：目的:研究耐药性癫痫患者脑组织中Bcl-2蛋白的表达,以探讨其在耐药性癫痫发生及发展中的作用。方法:收集153例耐药性癫痫患者术后脑组织标本,按随机化原则从中抽取20例作为本次研究的实验组,用免疫组织化学、免疫荧光,检测Bcl-2蛋白表达产物,按照配对设计原则与性别相同、年龄近似的对照组进行比较。结果:免疫组化及免疫荧光均可见Bcl-2蛋白在实验组中的表达量与对照组相同部位相比明显增加,t检验示实验组与对照组间的差异有统计学意义(P<0.05)。结论:耐药性癫痫患者脑组织中Bcl-2表达增高与在耐药性癫痫形成中起着重要作用的细胞凋亡有关,这种异常表达的蛋白质参与了耐药性癫痫的发生发展。Objective:To study the expression of Bcl-2 protein in the brain tissue of paitents with drug-refractory epilepsy and its clinical value. Methods:immunohistochemistry、immunofluorescence were used to detect the expression of Bcl-2 protein in the surgically removed brain tissue of patients with drug-refractory epilepsy from the brain bank of our department(n=20),and the results were compared with that of normal controls(n=20). Results:Bcl-2 protein expression was significantly increased in temporal lobe cortex and hippocampus of patients with drug-refractory epilepsy compared with controls in the same regions, and there was significant difference between patient group and control group by t-test. Conclusion:The results indicate that the overexpression of Bcl-2 in the cortex of temporal lobe and hippocampus may be involved in the pathophysiology of drug-refractory epilepsy.

关 键 词：BCL-2蛋白 癫痫 细胞凋亡 免疫组织化学 免疫荧光 

分 类 号：R742.1[医药卫生—神经病学与精神病学]