复方参七汤对胃、结直肠癌抑制作用的实验研究  

Experimental study of inhibitory effect of compound Shenqitang on gastric and colorectal carcinomas

作　　者：钱晶晶[1] 沈洪薰[1] 陆飞[1] 周树军[1] 陈玉泉[1] 

机构地区：[1]南通大学附属医院普外科,南通226001

出　　处：《南通大学学报（医学版）》2008年第5期332-335,共4页Journal of Nantong University(Medical sciences)

摘　　要：目的:研究复方参七汤治疗胃、结直肠癌的作用机制。方法:(1)复方参七汤对人胃癌细胞的体外抑制实验;(2)对MNNG诱发大鼠腺胃癌的体内抑制诱癌率实验;(3)对裸鼠结肠癌肝转移的体内抑制实验。结果:(1)复方参七汤对人胃癌BGC-823细胞株半数杀伤浓度(LC50)为0.048g/ml,随药物浓度增高,杀伤作用亦增强;半数抑制浓度(IC50)为0.007g/ml,随药物浓度增高,抑制有丝分裂指数逐渐减小,而抑制细胞集落形成亦增大;(2)复方参七汤对MNNG诱发大鼠腺胃癌发生率明显降低,实验组为6.9%(3/43)显著低于对照组58.9%(23/39),(P<0.01);对MNNG诱发大鼠腺胃癌组织中PCNA和CyclinD1阳性表达明显降低,实验组为13.88%(5/36)和22.86%(8/35),显著低于对照组75.76%(25/33)和62.86%(22/35),(P<0.01);(3)复方参七汤对人结肠癌LS174t细胞株接种裸鼠肝转移的肝转移数、肝转移癌重和肝癌重/肝重明显降低,实验组为2.42±0.99,63.67±21.29和8.90±3.10,显著低于对照组4.17±1.99,94.32±37.86和13.20±5.30(P<0.05);对裸鼠肝转移血中CK20mRNA表达的相对值减少,实验组为0.398±0.143显著低于对照组0.518±0.090,(P<0.05);对裸鼠肝转移组织中nm23H1-NDPK阳性表达呈过表达,实验组为66.67%(8/12)显著高于对照组16.6%(2/12),(P<0.05)。结论:复方参七汤在体外实验有杀伤癌细胞作用;在体内实验抑制大鼠对MNNG诱癌率;抑制裸鼠对人结肠癌细胞肝转移的作用。Objective:To investigate the mechanism of therapeutic effect of compound Shenqitang on the gastric and col-orectal carcinomas.Methods:Several experiments had been done,including:(1) the inhibitory effect of compound Shenqitang on human gastric adenocarcinoma cell line in vitro;(2) its inhibitory effects on Wistar rat gastric adenocarcinoma induced by MNNG;and(3) its inhibitory effect on the hepatic metastases of colorectal carcinoma in mice.Results:(1)Obvious inhibitory effects of compound Shenqitang had been found on the cell division and colony form of human gastric adenocarcinoma cell line BGC-823 in vitro.The LC50 was 0.007g crude drug/ml in colony form experiment.The drug had potent killing effect in higher concentration,with LC50 of 0.048g crude drug/ml;(2)The incidence of gastric adenocarcinoma in the experimental group(6.9%) was significantly lower than that in the control group(58.9%).The positive rates of PCNA and cyclin D1 in the experimental group were 13.88%(5/36) and 22.86%(8/35),which were significantly lower than those in the control group(P<0.01);(3)Compound Shenqitang inhibited hepatic metastases of colorectal carcinoma in mouse model.Nm23H1-NDPK positive expression rate(66.67%) was significantly higher in the experimental group than that(16.67%) in the control group(P<0.05).Conclusion:Compound Shenqitang can effectively kill the human gastric adnocarcinoma cell line BGC-823 in vit-ro,inhibit the incidence of the gastric adenocarcinoma induced by MNNG in Wistar rats,and inhibit hepatic metastasis of colon carcinoma in mouse model.

关 键 词：胃癌 复方参七汤 肝转移 

分 类 号：R735.2[医药卫生—肿瘤]