红车轴草提取物对小鼠T淋巴细胞体外活化的抑制作用  

Inhibitory effect of Trifolium pratense leguminosae extract on the in vitro activation of mouse T lymphocytes

作　　者：杨志[1] 曾耀英[1] 黄秀艳[1] 李林[1] 姚满林[1] 宋兵[1] 滕菲[1] 

机构地区：[1]暨南大学组织移植与免疫实验中心,广州510632

出　　处：《现代免疫学》2008年第5期377-380,共4页Current Immunology

基　　金："973"国家重大基础研究资助项目(2006CB504200;2004CB720100);广东省基金资助项目(2006B36030016);广州市科技局科技攻关重点资助项目(2006Z-E0091)

摘　　要：探讨红车轴草提取物(Trifolium pratense Leguminosae extract,TLE)对小鼠T淋巴细胞的体外活化的影响。无菌条件下制备小鼠淋巴细胞悬液;双色荧光抗体染色结合流式细胞术分别分析TLE对小鼠T淋巴细胞在刀豆蛋白A(ConA)或佛波醇酯(PDB)刺激下的体外分化抗原CD69、CD25、CD71表达的影响。终质量浓度为20、30、40 mg/L的TLE对小鼠T淋巴细胞在ConA或PDB刺激下的体外分化抗原CD69、CD25、CD71表达具有明显的抑制作用(P<0.01)。TLE对ConA或PDB刺激的不同时期的小鼠T淋巴细胞的体外活化具有明显的抑制作用。To investigate the influence of Trifolium pretense legumnosae extract(TLE) on the in vitro activation of mouse T lymphocytes,the mouse lymphocyte suspension was prepared under sterile condition and the effect of TLE on the expressions of the in vitro differential antigens CD69,CD25 and CD71 on T lymphocytes stimulated by ConA or PDB were analyzed by means of double-fluorescent staining plus flow cytometry.It was found that TLE at the mass concentration of 20,30 and 40 μg/ml showed marked inhibition on the expressions of CD69,CD25 and CD71 in T lymphocytes(P<0.01).This result indicates that TLE exhibits suppressive role on the in vitro activation of T lymphocytes.

关 键 词：红车轴草提取物 T淋巴细胞 CD69 CD25 CD71 

分 类 号：R392.12[医药卫生—免疫学]