细胞外信号调节激酶在脂多糖诱导内皮细胞β-1，4-半乳糖基转移酶-Ⅰ表达中的作用  

The role of ERK in LPS-induced β-1,4-galactosyltransferase-Ⅰexpression in endothelial cells

作　　者：贲志云[1] 程纯[1] 钱佶[1] 肖锋[1] 张福鹏[1] 季玉红[1] 

机构地区：[1]南通大学医学院微生物学与免疫学教研室,南通226001

出　　处：《南通大学学报（医学版）》2008年第6期430-432,共3页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(30770488)

摘　　要：目的:研究细胞外信号调节激酶(extracellular signal-regulated protein kinase,ERK)对脂多糖(LPS)诱导内皮细胞β-1,4-半乳糖基转移酶-Ⅰ(β-1,4-galactosyltransferase-Ⅰ,β-1,4-GalT-Ⅰ)表达的调节作用。方法:应用ERK通路抑制剂U0126预处理人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)2 h,再用LPS刺激HUVECs 4 h,分别用RT-PCR和Western-blot方法检测β-1,4-GalT-ⅠmRNA及蛋白水平表达变化,并通过内皮-单核细胞黏附试验观察HUVECs黏附能力的改变。结果:U0126显著抑制LPS引起的HUVECsβ-1,4-GalT-Ⅰ表达的上调及其黏附能力的上词。结论:ERK信号转导通路可能参与调节LPS诱导的内皮细胞β-1,4-GalT-Ⅰ的表达,并影响内皮细胞的黏附能力。Objective:To investigate the role of extracellular signal-regulated protein kinase(ERK)in regulating the expres- sion ofβ-1,4-galactosyltransferase-Ⅰ(β-1,4-GalT-Ⅰ)stimulated by lipapalysaccharide(LPS)in human umbilical vein en- dothelial cells(HUVECs).Methods:Cultured HUVECs were pretreated by U0126,an inhibitor of ERK for 2 hours,and then stimulated by LPS for 4 hours.The changes ofβ-1,4-GalT-ⅠmRNA expression were detected by RT-PCR and Western blotting,and adherence ability of HUVECs was observed by endot...

关 键 词：细胞外信号调节激酶 脂多糖 β-1 4-半乳糖基转移酶-Ⅰ 内皮细胞 

分 类 号：R363[医药卫生—病理学]