纳米微粒包装肿瘤抗原诱导CTL抗肿瘤免疫  

Nanoparticles formulated with tumor antigen induce the anti-tumor immue responses of cytotoxic T lymphocytes

作　　者：陈明水[1] 叶韵斌[1] 李洁羽[1] 周智锋[1] Boris Minev 

机构地区：[1]福建省肿瘤医院,福州350014 [2]University of California,San Diego

出　　处：《现代免疫学》2008年第6期441-446,共6页Current Immunology

摘　　要：用纳米微粒包装肿瘤抗原,并探讨其诱导产生CTL的免疫学特性和杀瘤活性。采用复乳溶剂蒸发法制备包含有恶性黑色素瘤抗原肽Mart-1-27-35纳米微粒;扫描电镜观察所制得纳米颗粒的形态。用NP-Mart-1-27-35诱导抗原特异性CTL并用ELISPOT法和LDH法检测其特性和杀瘤活性。结果:电镜观察所制得的纳米颗粒为圆形,平均直径为(208.52±12.43)nm。HPLC分析纳米-抗原肽的抗原载入量为6.72%±0.28%,平均包封率为91.23%±3.56%;ELISPOT检测表明纳米微粒包装的抗原肽能引起较强的免疫反应,产生分泌IFN-γ的细胞斑点数分别为(476.37±29.43)/2×104,显著高于单纯使用抗原肽时所产生的斑点数;LDH检测显示与单纯使用抗原肽相比,纳米-抗原肽诱导的CTL对Mart-1+细胞的杀伤能力显著提高。纳米微粒包装的抗原肽能够显著增强并延长DC对抗原的提呈作用;其诱导产生的CTL能以MHC I限制的方式特异识别和杀伤表达相应抗原的肿瘤细胞。To investigate the immunological characteristics and the killing effect of cytotoxic T lymphocytes(CTLs) on tumors with nanoparticles formulated with tumor antigen,nanoparticles containing Mart-1-27-35 peptide of malignant melanoma were prepared by using double emulsion solvent evaporation method.The shape and size of NP-Mart-1-27-35 particle was analyzed using scanning electron microscopy,and this particle was used to induce the antigen-specific CTLs.Its properties and the killing effect on tumor cells wer...

关 键 词：纳米 CTL 抗原肽 ELISPOT 

分 类 号：R730.5[医药卫生—肿瘤]