海洛因成瘾对大鼠PAG及海马神经元c-fos蛋白表达的影响  

The Expression of c-fos of PAG and Hippocampal Neurons in Heroin Addiction Rat

作　　者：秦伟[1] 秦继迅[2] 金寰[1] 肖智[1] 潘贵书[1] 

机构地区：[1]遵义医学院生理学教研室 [2]遵义医学院生物学教研室,贵州遵义563003

出　　处：《遵义医学院学报》2008年第5期450-453,共4页Journal of Zunyi Medical University

基　　金：遵义医学院中青年基金合同编号:院发(2006)006

摘　　要：目的探讨海洛因成瘾对大鼠中脑导水管周围灰质(periaqueductal gray,PAG)神经元及海马不同亚区神经元c-fos蛋白表达的影响。方法以剂量递增法皮下注射海洛因建立海洛因成瘾大鼠模型,腹腔注射纳洛酮诱发戒断症状。采用免疫组织化学法显示海洛因成瘾组大鼠与生理盐水对照组大鼠PAG神经元及海马神经元c-fos蛋白表达的差异。结果海洛因成瘾组大鼠PAGFos阳性细胞比纳洛酮催促戒断组大鼠和生理盐水对照组明显增多。双标染色显示Fos阳性细胞均聚集在PAG腹外侧。海洛因成瘾组大鼠海马CA1区和齿状回Fos阳性神经元数目明显增加(P<0.05),CA3区无明显改变(P>0.05)。纳洛酮催促戒断组大鼠海马CA1区和CA3区Fos阳性神经元数目明显增加(P<0.05),齿状回Fos阳性神经元数目也明显增加(P<0.05)。海洛因成瘾组大鼠与纳洛酮催促戒断组大鼠CA3区Fos阳性神经元数目有显著性差异(P<0.05)。结论PAG神经元及海马神经元c-fos蛋白表达增强可能与海洛因成瘾对神经元的损伤有关。Objective To study the difference of c-fos expression of neurons in subareas of periaqueductal gray and hippocampus of heroin addiction rat.Methods Rat were given increasing doses of heroin to form heroin addiction models and withdrawal syndromes were precipitated by naloxone.The expression of c-fos positive neurons in subareas of periaqueductal gray and hippocampus CA1,dentate gyrus and CA3 of hippocampus of heroin addiction group was observed by immunohistochemistry assay.Results The number of Fos positiv...

关 键 词：海洛因 成瘾 FOS蛋白 海马 PAG 

分 类 号：R74[医药卫生—神经病学与精神病学]