大鼠腹腔心脏异位移植模型的改进  

Improvement of a rat model of heart transplantation

作　　者：张晓慎[1] 吴若彬[1] 

机构地区：[1]广东省心血管病研究所心外科,广东广州510080

出　　处：《解剖学研究》2008年第6期452-454,共3页Anatomy Research

基　　金：广东省自然科学基金资助项目(7001109)

摘　　要：目的改进传统大鼠腹腔心脏异位移植模型,提高成功率。方法完全摘取供体心肺,离体供心灌注停跳;在松弛的状态下结扎静脉系统,端侧吻合采取间断+连续的缝合方法;先开放远心端再开放近心端,同时按摩心脏。结果传统方法组(n=20)成功14只,动脉吻合口出血2只、心脏复跳失败2只、吻合口狭窄2只;改进方法组(n=20)成功18只,2只死于吻合口出血。传统方法组手术成功率为70%,改进方法组为90%(P>0.05)。结论①快速摘取心肺,离体心脏灌注保护,操作简单,热缺血时间短,效果肯定,对供心的保护效果好。②松弛的状态下结扎静脉系统,对心房、冠脉影响非常小。③采取间断+连续的缝合方法,因吻合口狭窄造成手术失败的可能性很低,④合理定位,避免吻合完毕供心动脉扭曲,从而影响复跳。⑤注意受体鼠的保温、消毒及血容量丧失非常关键。⑥利用心外膜起搏导线获得了供心的动态心电图。Objective To improve the techniques for establishment of a stable and reproducible animal model of heart transplantation in rat for further research on the preservation of donor heart. Methods To obtain the donor`s heart and lung quickly at first. Then the hearts were arrested with HTK solution. To tie off all systemic and pulmonary inflow in a mass ligature without tension. End-toside anastomoses were performed from the donor ascending aorta to the recipient abdominal aorta and from the donor pulmonary art...

关 键 词：心脏移植 动物模型 腹腔 大鼠 

分 类 号：R-332[医药卫生]