应用组织芯片技术检测淋巴瘤患者EB病毒表达及意义  

To detect the EBV expression in lymphoma and its significance by the tissuechip technology

作　　者：力超[1] 郑雄伟[1] 赵文锦[1] 陆俐丽[1] 

机构地区：[1]福建省肿瘤医院病理科,350014

出　　处：《福建医药杂志》2008年第6期1-3,共3页Fujian Medical Journal

基　　金：福建省卫生厅青年科研课题资助项目(2004-1-8)

摘　　要：目的探讨福建地区淋巴瘤与EB病毒(EBV)感染的关系。方法应用组织芯片结合原位杂交技术检测121例淋巴瘤患者EB病毒的表达。结果霍奇金淋巴瘤(HL)EBER(epstein-barr virus early RNA,EBER)阳性者均为经典型HL,检出率为52.2%(24/46);HL各型中检出率以LD型最高,3例均为阳性,MC型检出率83.3%(15/18),NS型和LP型分别为35.7%(5/14)和14.3%(1/7);非霍奇金淋巴瘤(NHL)中T细胞淋巴瘤检出率40.0%(20/50),其中鼻型结外NK/TCL检出率高达100%(9/9);结内、结外T细胞淋巴瘤检出率分别为18.2%(6/33)、82.4%(14/17),两组之间EBV感染率有明显差异(P<0.01)。结论福建地区淋巴瘤的发病与EB病毒的感染有一定的关系,其中以HL中MC和LD及NHL中的T-NHL与EB病毒的关系较为密切。而且T细胞淋巴瘤EBV感染有部位和类型倾向性的特点。Objective To investigate the association between EBV infection and lymphoma in Fujian by tissue-chip.Methods Tissue-chip technique and hybridization in situ were performed on 121 cases of lymphoma to detect EBV.Results All EBV-associated Hodgkin s lymphoma(HL)were classical ones,24 of the 46 HL(52.2%)were showed EBER positive;The LD subtype were closely related to EBV infection,all of 3 LD subtype were EBER positive,while just 83.3% of MS subtype(15/18),35.7% of NS subtype(5/14)and 14.3% of LP subtype(1/7)w...

关 键 词：淋巴瘤 EB病毒 EBER 组织芯片 原位杂交 

分 类 号：R733.1[医药卫生—肿瘤]