实验性脑出血血肿周围脑组织基质金属蛋白酶组织抑制剂-1的表达及其与脑水肿的关系  

Expression of TIMP-1 in brain tissue of perihematoma and relationship between TIMP-1 and brain edema in experimental intracerebral hemorrhage

作　　者：赵辉[1] 李传玲[1] 王庆广[2] 崔桂云[3] 张艺潆[3] 陆军[3] 沈霞[3] 

机构地区：[1]徐州市中心医院神经内科,221009 [2]江阴市人民医院神经内科 [3]徐州医学院附属医院神经内科

出　　处：《临床神经病学杂志》2008年第6期431-433,共3页Journal of Clinical Neurology

基　　金：江苏省高校自然科学研究资助项目(05KJD320229)

摘　　要：目的探讨实验性脑出血(ICH)血肿周围脑组织基质金属蛋白酶组织抑制剂-1(TIMP-1)的表达及其与脑水肿的关系。方法采用自体血注入法建立大鼠ICH模型,制模后1h、3h、6h、12h、24h、36h、48h、72h、7d共9个时间点分别用干湿重法测血肿周围脑组织含水量,伊文思蓝(EB)检测血-脑屏障(BBB)通透性,Western Blot方法检测血肿周围组织的TIMP-1的表达;分析三者间的关系,并与假手术组相比较。结果与假手术组比较,ICH后各时间点血肿周围脑组织含水量和EB含量均明显增加(P<0.05～0.01),均于ICH48h达到高峰,且二者呈正相关(r=0.940,P<0.01);TIMP-1在ICH12～36h表达明显减低,ICH1～24h TIMP-1表达量与脑组织含水量以及EB含量呈负相关(r=-0.922,-0.950,均P<0.05)。结论大鼠ICH后血肿周围脑组织TIMP-1表达下调,可能促进了ICH早期的脑水肿形成。Objective To investigate the expression of TIMP-1 in brain tissue of perihematoma and the relationship between TIMP-1 and brain edema in experimental intracerebral hemorrhage(ICH). Methods ICH models of rats were established by autologous blood injection. Then the brain water content was measured by comparion of wet and dry weight,the permeability of blood-brain barrier (BBB) was evaluated by evans blue contents,and the expression of TIMP-1 in brain tissue of perihematoma was detected by Western Blot at 1 h...

关 键 词：脑出血 脑水肿 基质金属蛋白酶组织抑制剂-1 

分 类 号：R743.34[医药卫生—神经病学与精神病学]