高效液相色谱法测定穿心莲内酯掩味树脂复合物的含量  

Determination of Andrographolide in Resin Complexes of Andrographolide Ion-exchange Resin by HPLC

作　　者：孙艳荣[1] 王跃生[2] 梁玲[2] 刘芳菊[1] 杨帆[1] 丁丽[1] 欧阳旭[1] 

机构地区：[1]江西中医学院,江西南昌330006 [2]中药固体制剂制造技术国家工程研究中心,江西南昌330006

出　　处：《中国实验方剂学杂志》2008年第10期4-5,共2页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立HPLC法测定穿心莲内酯树脂复合物中穿心莲内酯含量的方法。方法:Hypersil C18柱,以甲醇-水(55∶45)为流动相,检测波长225 nm。结果:穿心莲内酯进样量在(0.095 6~0.956)μg范围内线性关系良好,回归方程为Y=230.4X+47.264,r=0.999 4(n=6),平均加样回收率为99.99%(n=9),RSD为0.88%。结论:该方法准确、可靠、重复性好,可作为穿心莲内酯树脂复合物中穿心莲内酯的含量测定方法。Objective:A HPLC method for determination of andrographolide s content in andrographolide-resin complexes was studied.Method:Hypersil C_18 column was used with methanol-water(55∶45) as the mobile and the detection wavelength was set at 225 nm.Results:linear in a range of 0.0956～0.956 μg the regression equation for andrographolide was Y=230.4X+47.264 with r=0.999 4.The average recovery was 99.99% and the RSD was 0.88%.Conclusion:The method is reliable and reproducible.It may be used for the determ ination of...

关 键 词：穿心莲内酯 掩味 树脂复合物 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]