E-cadherin、MCP-1和MIP-1β在B16黑色素瘤荷瘤鼠中的表达及意义  

Expression and significance of E-cadherin, MCP-1 and MIP-1β in B16 melanoma tissue

作　　者：纪长伟[1] 贾立敏[2] 倪秀芹[1] 鲍淑梅 朱保国[1] 

机构地区：[1]哈尔滨医科大学大庆校区,黑龙江大庆163319 [2]哈尔滨医科大学,黑龙江哈尔滨150081 [3]密山市人民医院,黑龙江密山158300

出　　处：《中国现代医学杂志》2008年第21期3103-3106,共4页China Journal of Modern Medicine

基　　金：黑龙江省教育厅课题(10551202)

摘　　要：目的检测皮肤B16黑色素瘤小鼠体内E-cadherin、MCP-1、MIP-1β的表达,探讨其与树突状细胞在体内迁移过程中的调控的关系。方法取C57纯系小鼠30只,分为12d肿瘤组,25d肿瘤组及正常对照组,每组10只。肿瘤组将B16黑色素瘤细胞人工植入C57小鼠背部皮下,制荷瘤鼠模型,采用免疫组织化学SABC法检测肿瘤病变中心区及交界区皮肤中E-cadherin、MCP-1、MIP-1β表达。结果肿瘤接种后第12天的MCP-1和MIP-1β在淋巴结和肿瘤中心区和交界区表达的面密度和数密度明显增高,分别与对照组和肿瘤第25天组比较差异有显著性(P<0.01)。肿瘤组的E-cadherin在淋巴结和肿瘤组织中表达降低,第25天组尤其明显;第25天组与第12天组E-cadherin表达分布面密度和数密度比较差异有显著性(P<0.01),第12天组和对照组比较差异有显著性(P<0.01)。结论在肿瘤状态下,E-cadherin、MCP-1和MIP-1β有促进树突状细胞向淋巴组织和肿瘤局部迁移和聚集的作用。E-cadherin、MCP-1、MIP-1β的异常表达在皮肤黑色素瘤的恶性进展过程中起重要作用。Objectives To study the expression of E-cadherin, MCP-1 and MIP-1β in B16 melanoma mouse models, and to investigate their role in tumor invasion and metastasis.Methods30 cases C57 mice were divided into 3 groups:12 d tumor group, 25 d tumor group and normal control group.They were injected subcutaneously at left dorsal skin with B16 melanoma cells.Skin around and in the center of tumor and axillary lymph nodes were removed for HE staining and immunohistochemistry.ResultsThe difference of quantity density an...

关 键 词：E-CADHERIN MCP-1 MIP-1Β 黑色素瘤 

分 类 号：R739.5[医药卫生—肿瘤]