诱导分化药物对肺癌细胞肿瘤相关基因的影响  

Study on expression of genes in lung cancer cells A549 treated by inducer of differentiation

作　　者：陈侃[1] 赵俊刚[2] 石文君[2] 纪子钊[2] 

机构地区：[1]辽宁锦州市中心医院胸外科,辽宁锦州121004 [2]中国医科大学盛京医院胸外科,辽宁沈阳110001

出　　处：《中国现代医学杂志》2008年第22期3266-3268,3272,共4页China Journal of Modern Medicine

摘　　要：目的通过观察诱导分化浓度的全反式维甲酸、亚硒酸钠、三氧化二砷对肺腺癌细胞A549的肿瘤相关基因的影响,探讨诱导分化药物对肺癌细胞的作用机制。方法体外培养肺癌A549细胞,设全反式维甲酸、亚硒酸钠和三氧化二砷处理组及对照组,药物处理24h后以Western Blot和Rt-PCR检测基因caspase3、p21、RARα、bcl-2、cyclinD3和nm23的表达。结果caspase3、RARα和nm23在亚硒酸钠、三氧化二砷组中表达上调;p21在全反式维甲酸、三氧化二砷组中表达上调;bcl-2和cyclinD3在3个处理组中表达下调。结论全反式维甲酸、亚硒酸钠和三氧化二砷能引起A549肿瘤相关基因表达的改变,诱导分化药物对肺癌的作用机制表现为增殖抑制、促进凋亡、周期阻滞、转移抑制、诱导分化敏感度增加等多方面的改变。【Objective】To investigate the mechanism of induction differentiation by studying the expression of Genes in lung cancer cells A549 treated by induer of differentiation.【Methods】 The lung cancer cells were cul-tured and treated by All-trans Retinoic acid,Sodium selenite and Arsenic trioxide at the concentration of 5.0 μmol/ L,respectively.The western blot and the Rt-PCR method was used to detect the expression of caspase3,p21,RARα,bcl-2,cyclinD3 and nm23.【Results】The expression of anti-oncogenes caspase3,p21...

关 键 词：A549细胞 诱导分化 基因表达 

分 类 号：R734.2[医药卫生—肿瘤]