增龄和去睾丸大鼠不同结构骨组织的变化及雄激素的干预作用  

Effects of testosterone on different part of skeletal metabolism in orchidectomized rats

作　　者：苏开鑫[1] 陈艳[1] 谢华[1] 王宏芬[1] 

机构地区：[1]广东医学院,广东湛江524023

出　　处：《中国现代医学杂志》2008年第22期3286-3289,共4页China Journal of Modern Medicine

摘　　要：目的探讨增龄和去睾丸对成年大鼠不同结构骨组织骨代谢的影响,并观察雄激素的干预作用效果。方法40只4个月龄SD雄性大鼠,随机分成基础对照组(A组)、年龄对照组(B组)、去睾丸组(C组)和去睾丸加睾丸酮组(D组),基础对照组在实验开始时处死,其余同等条件下饲养90d后,处死全部大鼠,取腰椎、胫骨上段和中段进行不脱钙骨包埋切片,应用计算机自动图像分析系统进行骨组织形态计量学分析。结果年龄对照组大鼠胫骨上段骨形成和骨吸收均下降,出现明显的骨质丢失。而去睾丸组大鼠胫骨上段骨量下降,骨形成和骨吸收参数都增加;胫骨中段皮质骨静态参数截面总面积、髓腔面积等无明显变化,动态参数骨外膜骨形成降低,骨内膜的骨形成和骨吸收均有增加趋势。去睾丸加睾丸酮组大鼠胫骨上段的骨量比去睾丸组增加,骨高转换率降低;胫骨中段皮质骨骨外膜的骨形成增加,骨内膜的骨吸收降低(P<0.05)。结论增龄使大鼠会出现明显的骨质丢失,而去睾丸后骨质在增龄的基础上进一部丢失,以松质骨较为显著,皮质骨变化较慢。补充睾丸酮后可以阻止由于增龄和去睾丸所引起的骨质丢失,并能维持正常的骨质结构。【Objective】 To study the effects of testosterone on different part of skeletal metabolism in ORX rats by bone histomorphometry.【Methods】Forty four-month-old Sprague Dawley male rats were randomly divided into four groups:basal group (group A),age control group (group B),ORX group (group C) and ORX with methyltestos-terone group (group D).Group A were killed at the begin of the study.After 90 days,all rats were killed and their proximal tibia and tibial diaphysis were processed undecalcified sections and mea...

关 键 词：骨形态计量学 雄激素 去睾丸 松质骨 皮质骨 

分 类 号：R[医药卫生]