丹参酮ⅡA与5-FU抑制HeLa细胞生长及诱导细胞凋亡的研究  

Study on apoptosis and inhibited growth of human cervical carcinoma HeLa cell induced by TanshinonⅡ A and 5-FU

作　　者：王冬[1] 田亚平[1] 姜英雁[1] 任宝红[1] 方青[1] 李英女[1] 

机构地区：[1]吉林医药学院附属医院妇科,吉林吉林132011

出　　处：《中国现代医学杂志》2008年第22期3237-3240,共4页China Journal of Modern Medicine

摘　　要：目的探讨丹参酮ⅡA与5-氟尿嘧啶(5-FU)对宫颈癌HeLa细胞增殖抑制及诱导细胞凋亡的影响。方法采用MTT方法、荧光染色、流式细胞仪及电镜技术等研究丹参酮ⅡA与5-FU对HeLa细胞增殖抑制及诱导细胞凋亡作用。结果丹参酮ⅡA对宫颈癌HeLa细胞增殖有明显抑制作用,并呈现浓度和时间的依赖性。与5-FU合用后细胞增殖抑制率明显增强。8.0μg/mL丹参酮ⅡA作用72h后,电镜下出现细胞核固缩,形成凋亡小体。流式细胞仪检测丹参酮ⅡA4.0和8.0μg/mL作用HeLa细胞72h后细胞凋亡率为34.13%、45.84%,在浓度增至32.0μg/mL时细胞凋亡无明显增加。结论丹参酮ⅡA具有直接抑制宫颈癌HeLa细胞增值作用且在一定浓度范围内诱导细胞凋亡,同时具有增强5-FU的抗肿瘤作用。【Objective】To investigate the regulatory effect of Tanshinon ⅡA on proliferation and apoptosis of human cervical carcinoma Hela cells in vitro with 5-FU.【Methods】 Inhibition of cervical carcinoma Hela cell growth by Tanshinon ⅡA and 5-FU was determined by TMM assay and growth curve,and apoptosis was detected by electron microscopy and flow cytometry (FCM).【Results】Tanshinon ⅡA showed dose-dependent and time-de-pendent inhibitory effects on the proliferation in cervical carcinoma Hela cell line.After treatme...

关 键 词：丹参酮ⅡA 5-FU 宫颈癌 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]