8-硝基白杨素诱导人结肠癌HT-29细胞凋亡  

Induction of apoptosis of human colon cancer HT-29 cells by 5,7-Dihydroxy-8-nitrochrysin

作　　者：伍尤华[1] 曹建国[2] 

机构地区：[1]南华大学附属第一医院肿瘤内科,湖南衡阳421001 [2]南华大学医学院肿瘤研究所,湖南衡阳421001

出　　处：《中国现代医学杂志》2008年第23期3443-3445,共3页China Journal of Modern Medicine

基　　金：湖南省自然科学基金(No:03JJY5009);湖南省卫生厅医药科研项目(No:B2006-141;C2005-036)

摘　　要：目的观察8-硝基白杨素(NOChR)诱导人结肠癌HT-29细胞凋亡作用。方法MTT比色法测定细胞增殖活性;流式细胞分析术检测细胞凋亡率;琼脂糖凝胶电泳观察DNA梯形条带。结果NOChR抑制人结肠癌(HT-29)细胞增殖,呈浓度依赖性,其IC50值为1.78μM;NOChR具有诱导HT-29细胞凋亡作用;PPARγ特异性阻断剂(GW9662)能有效拮抗NOChR诱导HT-29细胞凋亡作用。结论NOChR诱导HT-29细胞凋亡作用与其活化PPARγ相关。ObjectiveTo investigate induction of apoptosis of human colon cancer HT-29 cells by 5,7-dihydrox-8-nitrochrysin in vitro. MethodsThe proliferative activity of HT-29 cells treated with 5,7-dihydrox-8-nitrochrysin was measured using 3-(4,5-dimethylthiazol-2-yl) -2,5-dipheny tertrazolium blue(MTT) colorimetric assay. HT-29 cell apoptotic percentage was detected by flow cytometry with PI staining. DNA ladder bands were observed by DNA agarose gel electrophoresis. ResultsMTT assay suggested that 5,7-dihydrox-8-n...

关 键 词：结肠癌 白杨素 白杨素衍生物 凋亡 

分 类 号：R735.35[医药卫生—肿瘤]