Ph1染色体及bcr/abl融合基因检测在慢性粒细胞白血病中的诊断意义  

Significance of detection of Ph1 chromosome and bcr/abl fusion gene in chronic myelocytic leukemia

作　　者：曹友德[1] 徐伟[1] 肖佩玲[2] 石詠中[1] 聂盛丹[1] 付冀辉[2] 

机构地区：[1]湖南省人民医院临床医学研究所,湖南长沙410005 [2]湖南省人民医院血液科,湖南长沙410005

出　　处：《中国现代医学杂志》2008年第23期3520-3522,共3页China Journal of Modern Medicine

摘　　要：目的探讨Ph1染色体和bcr/abl融合基因检测在慢性粒细胞白血病诊断中的意义。方法选择28例慢性粒细胞白血病患者和20例缺铁性贫血患者作为研究对象,采用骨髓细胞短期培养法制备染色体、采用RT-PCR法分析bcr/abl融合基因。结果28例慢性粒细胞白血病患者中,Ph1染色体阳性者25例(89.3%),bcr/abl阳性者26例(92.9%),Ph1和bcr/abl均阳性25例(89.3%),Ph1阴性、bcr/abl阳性1例(3.6%),Ph1和bcr/abl均阴性2例(7.1%)。20例缺铁性贫血患者Ph1和bcr/abl均阴性。结论Ph1染色体、bcr/abl融合基因的检测有助于慢性粒细胞白血病的诊断和鉴别诊断。ObjectiveTo explore the clinical significance of detection of Ph1 chromosome and bcr/abl fusion gene in the diagnosis for chronic myelocytic leukemia(CML) . MethodsTwenty-eight patients with chronic myelocytic leukemia and twenty patients with iron deficiency anemia were selected. Bone marrow short-term culture was used to prepare the chromosome,bcr/abl fusion gene was analyzed by RT-PCR method. ResultsIn 28 patients with chronic myelocytic leukemia,25(89.3%) cases were Ph1 Chromosome positive,26(92.9%) cas...

关 键 词：白血病 Ph1染色体 BCR/ABL融合基因 

分 类 号：R733.72[医药卫生—肿瘤]