大鼠心肌细胞低温下微管改变与冷缺血损伤相关性的研究  

Study on correlationship between change of microtubules of cardiomyocytes and cold ischemic injury in hypothermia in rat

作　　者：孙长滨[1] 杨春光[2] 孙桂媛 

机构地区：[1]大连大学医学院组织胚胎学教研室,辽宁大连116622 [2]大连大学医学院药理学教研室,辽宁大连116622 [3]中国医学大学组织胚胎学教研室,辽宁沈阳110001

出　　处：《中国现代医学杂志》2008年第24期3594-3597,共4页China Journal of Modern Medicine

摘　　要：目的研究4℃环境下心肌细胞微管的变化与冷缺血损伤之间的关系。方法常规方法分离SD成年大鼠心肌细胞,随机分成对照组(单纯KB液保存)和实验组(KB液中含紫杉酚,浓度为10-6M),利用免疫荧光的方法检测各组在4℃条件下,分别保存0.5和3h后的微管网络结构的变化,同时利用生物化学比色法检测保存液中乳酸脱氢酶(LDH)含量,台盼蓝染色法显示细胞存活百分比。结果经历4℃保存后,实验组微管平均光密度明显高于对照组,实验组微管网络形态分布比对照组完整,实验组乳酸脱氢酶释放量低于对照组,台盼蓝染色显示实验组细胞存活率高于对照组。结论4℃环境下微管的解聚与心肌细胞损伤关系密切,稳定微管能减轻冷缺血引起的细胞损伤。[Objective] To investigate the correlationship between the change of microtubules of cardiomyocytes and cold ischemic injury under 4℃ preservation.[Methods] The cardiomyocytes separated from SD rat with the routine methods were divided randomly into control group(preserved in KB solution) and experimental group(appending taxol at 10-6 M in KB solution) and preserved for 0.5 h or 3 h under 4℃.Then the changes of microtubular network were detected with immunofluorescence,the content of LDH in preservation sol...

关 键 词：心肌细胞 微管 低温 冷缺血 

分 类 号：R363[医药卫生—病理学]