结缔组织生长因子反义寡核苷酸对肝星状细胞胶原表达的影响  

Effects of antisense oligonucleotide of Connective tissue growth factor gene on the expression of procolleganα_2(Ⅰ) in HSC-T_6

作　　者：梁增文[1] 张国[1] 农兵[1] 

机构地区：[1]广西壮族自治区人民医院消化内科,南宁530021

出　　处：《陕西医学杂志》2008年第11期1443-1446,共4页Shaanxi Medical Journal

基　　金：广西科学研究与技术开发项目(NO:0448030)

摘　　要：目的:观察结缔组织生长因子(CTGF)反义寡核苷酸对肝星状细胞株(HSC-T6)前胶原α2(Ⅰ)表达的影响。方法:实验分为反义寡核苷酸(ASODN)组、正义寡核苷酸(SODN)组、脂质体对照组、空白对照组。除空白对照组外,其余各组均于转导前提前加入TGF-β1因子刺激。结果:ASODN能显著抑制经TGF-β1活化后的HSC-T6前胶原α2(Ⅰ)mRNA的表达,而SODN和脂质体对照组对HSC-T6前胶原α2(Ⅰ)mRNA的表达均无明显影响。结论:前胶原α2(Ⅰ)表达与CTGF密切相关,进一步提示CTGF可能是肝纤维化发生发展的关键介导者;应用反义寡核苷酸封闭CTGF的表达有望成为肝纤维化治疗的一种新手段。Objective:To observe the effects of antisense oligonucleotide(ASODN)of Connective tissue growth factor gene on the expression of procollegan α2(Ⅰ)in HSC-T6.Methods:Cultured cells were divided into four groups:ASODN,sense oligodeoxynucleotide(SODN),liposome control and blank control.Except for the blank control,all other groups were stimulated by TGF-β factor prior to adding oligodeoxynucleotide.Results:Stimulated by TGF-β,the CTGF mRNA and procollegan α2(Ⅰ)mRNA in HSC-T6 were significantly down-regulated or...

关 键 词：肝硬化/免疫学 @结缔组织生长因子 @肝星状细胞 基因表达 胶原Ⅰ型/免疫学 寡核苷酸类 反义 

分 类 号：R363[医药卫生—病理学]