定量测定45例血小板膜糖蛋白及其在急性脑梗死中的意义  

Quantitative analysis of platelet membrane glycoprotein and its clinical significance in acute cerebral infarction

作　　者：江雁[1] 穆红[1] 唐志琴[1] 黄繁嫱[1] 

机构地区：[1]天津市第一中心医院检验中心,天津300192

出　　处：《陕西医学杂志》2008年第12期1672-1674,共3页Shaanxi Medical Journal

摘　　要：目的:探讨流式细胞术定量测定血小板膜糖蛋白在急性脑梗死中的临床意义。方法:应用流式细胞仪测定静息状态和TRAP激活的血小板所结合单抗的分子数。结果:急性脑梗死患者血小板静息状态下GPⅢa和GPⅡb/Ⅲa密度[分别为(76.8±14.1)×103/血小板、(73.9±10.5)×103/血小板]高于健康对照组[分别为(55.6±5.8)×103/血小板、(50.5±4.1)×103/血小板,P<0.05],TRAP活化后患者血小板GPⅢa和GPⅡb/Ⅲa密度[分别为(102.6±16.4)×103/血小板、(108.5±16.7)×103/血小板]亦明显高于对照组[分别为(69.7±18.3)×103/血小板、(61.2±5.7)×103/血小板,P<0.01];患者血小板静息状态下,GMP-140表达与对照组比较没有显著性差异(P>0.05),而TRAP激活后患者血小板GMP-140表达[(1.21±0.41)×103/血小板]明显高于对照组[(0.87±0.26)×103/血小板,P<0.05];患者血小板静息和活化状态GPⅠb表达与对照组比较没有显著性差异(P>0.05)。结论:急性脑梗死患者血小板呈较为活化状态,血小板膜糖蛋白测定对病情的分析和疗效的判断有重要的临床参考价值。Objective:To explore the flow cytometric quantitative analysis of platelet membrane glycoprotein and its clinical significance in acute cerebral infarction.Methods:The molecule numbers of platelet GPⅠb,GPⅢa,GPⅡb/Ⅲa and GMP-140 on resting platelets and platelets activated by TRAP were detected by Flow cytometry.Results:Whether on resting platelets or platelets activated by TRAP,the surface densitits of GPⅢa and GPⅡb/Ⅲa in patients with cute cerebral infarction were significantly higher than those in healthy ...

关 键 词：脑梗塞/诊断 血小板膜糖蛋白类 流式细胞术 

分 类 号：R743.3[医药卫生—神经病学与精神病学]