表没食子儿茶素没食子酸酯逆转多药耐药肝癌细胞株基因表达谱变化  

Impact of (-) Epigallocatechin-3-gallate on gene expression profiles of human MDR hepatocellular carcinoma cell line

作　　者：唐海桦[1] 周铭[1] 张海英[2] 梁钢[1] 

机构地区：[1]广西医科大学药学院药理教研室 [2]广西医科大学公共卫生学院,南宁530021

出　　处：《中药药理与临床》2008年第5期14-18,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：广西科技厅自然科学基金资助项目(No.0447043)

摘　　要：目的:采用基因芯片技术获取表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)逆转肝癌多药耐药(multi-drug resistance,MDR)细胞前后的基因表达谱变化,探讨EGCG的可能耐药逆转作用机制。方法:cDNA微阵列分析EGCG作用肝癌MDR细胞株BEL7404/ADM前后的基因表达谱差异,Western Blot法检测细胞Cyclin G1蛋白表达变化以验证基因芯片分析的结果。结果:相对BEL7404/ADM细胞组,EGCG作用后细胞双荧光通路显著差异基因210个,上调表达38个,下调表达172个。与EGCG可能逆转耐药相关的基因有ABCB10(MDR/TAP),TOP2A,TOP2B,CCNG1上调,ABCB1,MVP,ARHD,HDAC5,GSS,GSTPI,HSPA1B,HSPB7,CDKN1A,RAB11B,RAB9P40下调等。Cyclin G1蛋白表达增加,符合cDNA微阵列基因水平变化。结论:EGCG多基因、多环节、多途径改变参与逆转ADM诱导的肝癌MDR,Western blot与基因芯片分析结果一致。Aim: To investigate the mechanism of the MDR-reversing effect of EGCG.The MDR-expressing human hepatocelluar carcinoma cell line,BEL7404/ADM was studied using cDNA microarrays to obtain gene expression profiles.Methods: cDNA microarrays were used to analyse differences in gene expressions in MDR hepatocellular carcinoma cell line with or without EGCG.The expression of Cyclin G1 protein in MDR HCC cell line was measured by Western blot to confirm the result of the cDNA microarray analysis.Results: There were...

关 键 词：表没食子儿茶素没食子酸酯 基因芯片 肝细胞癌 多药耐药性 

分 类 号：R285[医药卫生—中药学]