几种不同实验设计在低分子肝素活性测定中的比较  

Several experimental designs for evaluating the bioactivity of low-molecular-weight heparin

作　　者：赵慧玲[1] 

机构地区：[1]国家食品药品监督管理局药品审评中心,北京100038

出　　处：《中国临床药理学与治疗学》2008年第9期1024-1028,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:对低分子肝素(LMWH)生物活性测定中几种不同的实验设计进行比较。方法:对LMWH生物活性测定采用酶促反应的分光光度法,进行了量反应平行线实验设计中4.4法与4.3法、3.4法、3.3法的比较。结果:量反应平行线实验设计中4.4法与4.3法、3.4法、3.3法结果相似,其差异无统计学意义(P>0.05)。结论:采用酶促反应的分光光度法对LMWH的效价测定,应采用量反应平行线法实验设计;在符合标准规定前提下,4.4法与4.3法、3.4法、3.3法不同剂量组实验设计都可应用。AIM: To compare several experimental designs for detecting the bioactivity of low-molecular-weight heparin(LMWH).METHODS: Chromogenic assay was used for detecting the bio-activity of LMWH by comparing the 4·4 method to 4·3,3·4 or 3·3 method in quantity response parallel line design.RESULTS: In bioassay of LMWH with the quantity response parallel line analysis,there had no statistical difference(P>0.05) between 4·4 method with 4·3,3·4 or 3·3 method.CONCLUSION: Quantity response parallel line design could ...

关 键 词：低分子肝素 实验设计 生物统计 生物测定 

分 类 号：R927[医药卫生—药学]