CDKN2/p16基因HapⅡ位点甲基化与肺癌关系的研究  

Effect of methylation at Hap Ⅱ locus of CDKN2/p16 gene on lung carcinogenesis

作　　者：党伟[1] 张娣[1] 果应菲[1] 赵晓东[1] 孙荣距[1] 

机构地区：[1]解放军总医院第一附属医院急救部,北京100037

出　　处：《感染．炎症．修复》2008年第4期210-212,257+213,共5页Infection Inflammation Repair

基　　金：国家自然科学基金资助项目(30700869)

摘　　要：目的:通过检测CDKN2/p16抑癌基因上游调控序列及外显子E1 HapⅡ位点甲基化状态,探讨基因甲基化在肺癌发生中的作用,为肺癌的早期诊断及基因治疗提供可能的理论依据。方法:取58例肺癌患者手术切除的肺癌病理组织标本,16例肺组织良性病变并手术患者的病理标本作为对照。免疫组织化学技术检测p16蛋白表达,甲基化特异性聚合酶链反应(MS-PCR)技术分析该基因HapⅡ位点甲基化状态。结果:58例肺癌患者中,14例HapⅡ位点发现甲基化,甲基化率为24.14%,而对照组没有发现甲基化。其中,p16蛋白阳性者中2例发现甲基化,甲基化率为7.4%,而p16蛋白阴性者中12例发现甲基化,甲基化率为38.7%,差异有显著性(X^2=7.72,P=0.006)。结论:CDKN2/p16基因外显子E1及调控序列HapⅡ位点异常甲基化,可抑制p16蛋白表达,这可能是p16基因功能丧失的一个重要机制,并可能对肺癌发生起促进作用。Objective:To explore alternative mechanisms of cyclin-dependent kinase inhibitor 2(CDKN2)/p16 gene inactivation in human lung cancers and suggest a hypothesis of early detection and gene therapy by detecting HapⅡlocus aberrant methylation status of p16 gene upstream region and exon 1.Methods:The cancer tissue was obtained from 58 patients with lung cancer,and lung tissue was also obtained from 16 patients with lung benign disease of the lung.p16 gene and protein expression were detected by immunohistochemis...

关 键 词：肺癌 甲基化特异性PCR CDKN2/p16抑癌基因 甲基化 

分 类 号：R734.2[医药卫生—肿瘤]