苯乙酸对胶质瘤C_6细胞中Bcl-2、Bax和Caspase-3表达的影响  

Effect of Phenylacetate on the Expression of Bcl-2,Bax and Caspase-3 in Rat C_6 Glioma Cells

作　　者：杨朝华[1] 李国平[1] 游潮[1] 黄少伟[2] 韩方华[2] 姚业轩[2] 

机构地区：[1]四川大学华西医院神经外科,四川成都610041 [2]广西北海市人民医院神经外科,广西北海536000

出　　处：《华西医学》2008年第6期-,共2页West China Medical Journal

基　　金：广西科学基金资助项目(桂科青0640078)

摘　　要：目的:观察诱导分化剂苯乙酸(phenylacetate,PA)对Bcl-2、Bax和Caspase-3表达的影响,探讨PA诱导胶质瘤C6细胞凋亡的机制。方法:体外培养胶质瘤C6细胞,实验组给予PA浓度5.0mmol/L诱导72h,对照组不用PA,用原位杂交检测Bcl-2、Bax和Caspase-3mRNA的表达,图像分析比较表达水平的差异。结果:两组比较,Bcl-2无明显变化(P>0.05),实验组的Bax和Caspase-3表达增强(P<0.01)。结论:PA能增强Bax和Caspase-3的表达,其诱导胶质瘤C6细胞凋亡的机制可能与此有关。Objective:To observe the effect of differentiation inducer phenylacetate(PA)on the expression of Bcl-2,Bax and Caspase-3 in rat C6 glioma cells and explore the possible mechanisms of PA action.Methods:C6 cells were cultured in vitro.In experiment group,C6 cells were induced by PA at concentration of 5.0 mmol/L for 72 h.In control group,no PA was administrated.The expression of Bcl-2,Bax and Caspase-3 were detected in situ by hybridization.Results:The expression of Bcl-2 was unchanged in the two groups(P>...

关 键 词：苯乙酸 胶质瘤 凋亡 

分 类 号：R739.4[医药卫生—肿瘤]