β-连环蛋白和E-钙粘蛋白在小鼠磨牙牙胚发育中的研究  

Immunohistochemical research of β-catenin and E-cadherin in the developing mouse molar

作　　者：王淑霞[1] 刘大勇[1] 刘士有[1] 

机构地区：[1]天津医科大学口腔医院牙体牙髓科,300070

出　　处：《现代口腔医学杂志》2009年第1期70-73,共4页Journal of Modern Stomatology

摘　　要：目的研究β-连环蛋白(β-catenin,β-cat)和E-钙粘蛋白(E-cadherin,E-cad)在小鼠磨牙牙胚发育过程中的表达分布和意义。方法取胚胎13,15,16天(E13,E15,E16)和出生后1天、5天(P1,P5)的ICR小鼠,制备含上颌第一磨牙的各阶段牙胚发育标本,免疫组化二步法检测β-cat和E-cad在牙胚发育不同时期的表达。结果E13天牙胚处于蕾状期,E15天为帽状期,E16天为钟状早期,P1天处于钟状中期,P5天为钟状晚期。β-cat和E-cad在牙齿发育的各个阶段表达具有时空特异性,前者在各个阶段的成釉器上皮和其下方间充质中均表达,后者在釉质形成之前仅表达于成釉器上皮,之后在成牙本质细胞中也有表达。结论β-连环蛋白和E-钙粘蛋白参与牙胚发育的细胞形成与分化,表明细胞粘附和Wnt信号途径对牙胚发育具有调控作用。Objective To study the expression of β-catenin and E-cadherin in the different stages of developing mouse molar.Methods ICR mice of embryonic day 13,15,16 and postnatal day 1 and 5 were obtained.Tissues including the maxillary molar germs were used to get the specimens.Immunohistochemical staining was performed at the specimens of different stages.Results At embryonic day 13,15,16 and postnatal day 1,5,the first molar germs were at the bud stage,cap stage,early bell stage,mid bell stage and late bell stage ...

关 键 词：Β-连环蛋白 E-钙粘蛋白 牙齿发育 免疫组化 

分 类 号：R78[医药卫生—口腔医学]