Aβ对大鼠血管平滑肌细胞的影响及依达拉奉干预作用的研究  

Effect of Aβ on the vascular smooth muscle cells of rat and interventional study of Edaravone

作　　者：安俊[1] 刘佳[1] 刘昌勤[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经科,武汉430022

出　　处：《脑与神经疾病杂志》2009年第1期20-23,共4页Journal of Brain and Nervous Diseases

摘　　要：目的观察Aβ对大鼠血管平滑肌细胞APP基因表达的改变,探讨Aβ对血管平滑肌细胞(VSMC)的损伤作用;研究依达拉奉对上述过程的干预作用。方法体外培养VSMC,对照组用培养基培养,A组用Aβ和VSMC共同培养,B组用依达拉奉和Aβ与VSMC共同培养。光镜下观察各组细胞形态学变化。用MTT测各组细胞存活率,流式细胞仪测细胞凋亡率,RT-PCR测细胞APP基因的表达水平。结果1.光镜下可见,对照组细胞形态典型,数量均匀;A组细胞数量明显减少;B组细胞数量较A组增加。2.细胞存活率:A组细胞存活率较对照组降低(P<0.05),B组较A组增加(P<0.05)。3.细胞凋亡率:A组细胞凋亡率较对照组增加(P<0.05),B组较A组降低(P<0.05)。4.APP基因表达:A组细胞APP基因表达较对照组增加(P<0.05),B组细胞APP基因表达与A组没有明显统计学差异(P>0.05)。结论Aβ损伤VSMC,导致细胞存活率下降,凋亡增加,上调APP基因的表达,依达拉奉可以减轻Aβ对细胞的损伤,但对APP基因的表达上调没有影响。Objective To observe the alteration of the expression of APP gene caused by Aβ in VSMC of rat,explore the injury of Aβ to the VSMC and investigate the interventional effect of Edaravone on that process. Methods Vascular VSMC of rat were cultured in vitro. Control group:SMC were cultured with substratum. Group A :VSMC were cultured with Aβ and substratum.Group B:VSMC were cultured with Aβ,substratum and Edaravone. Morphologic changes of VSMC was observed under the microscope. Viability of VSMC was detected b...

关 键 词：β淀粉样蛋白(Aβ) 血管平滑肌细胞(VSMC) 凋亡 淀粉样前体蛋白(APP)基因 依达拉奉 

分 类 号：R743.3[医药卫生—神经病学与精神病学]