快速检测牛奶中大肠杆菌的压电生物传感器研究  

Construction and Application of Piezoelectric Biosensor for Rapid Detection of Escherichia coli in Milk

作　　者：刘素芹[1] 戴高鹏[1] 王启会[1] 

机构地区：[1]襄樊学院化学与生物科学系,湖北襄樊441053

出　　处：《食品科学》2009年第4期207-209,共3页Food Science

摘　　要：构建了一台基于串联式压电传感器的自动化微生物检测仪。对加入大肠杆菌的模拟牛奶样品进行检测,根据检测时间和样品中细菌浓度之间的线性关系,可以对样品中的细菌浓度进行计数,测定范围为10个/ml到106个/ml,检测时间3～15h。该方法的准确度与标准的平板计数法相当(r≥0.99),但是更灵敏、快速、简便。An automatic detection instrument based on tandem type piezoelectric biosensor was established,and then a model milk sample inoculated with Escherichia coli was tested by this instrument.Based on the linear relationship between frequency detection time(FDT) and bacterial concentration of milk sample,accurate numbers of counting bacteria were attainable.The results showed that target bacteria may be detected at concentrations ranged from 10 cell/ml to 106 cell/ml,and detection time is 3 to 15 h.The accuracy ...

关 键 词：压电传感器 大肠杆菌 牛奶 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]