人乳腺癌MCF-7/ADM细胞的耐药谱及brca1基因表达的实验研究  

Study drug-resistant spectrum of MCF-7/ADM and expression of brca1

作　　者：孙晶琳[1] 杨杨[1] 娜琴[1] 迟小溪[1] 包金兰[1] 矣方圆[1] 沙晶[1] 刘矩[1] 孟庆阳[1] 祁兴岩[1] 胡军[2] 

机构地区：[1]大连医科大学 [2]大连医科大学组织胚胎学教研室,辽宁大连116044

出　　处：《大连医科大学学报》2009年第1期58-60,共3页Journal of Dalian Medical University

基　　金：辽宁省教育厅科学研究计划资助(2005134)

摘　　要：[目的]研究人乳腺癌MCF-7/ADM细胞的耐药谱并探讨brca1基因产物表达与该细胞耐药性的相关性。[方法]经MTT法检测MCF-7/ADM细胞的耐药谱;通过免疫荧光法检测brca1基因产物表达情况。[结果]测得MCF-7/ADM细胞对阿霉素的耐药倍数为3.7倍;对长春新碱的耐药倍数为70.3倍;对氟尿嘧啶的耐药倍数为7.8倍。brca1基因产物在MCF-7/ADM细胞中的表达强度(98.23±7.63)高于MCF-7/S细胞(8.69±1.25),差异具有显著性意义(P<0.01)。[结论]MCF-7/ADM细胞是多药耐药细胞株,brca1与肿瘤多药耐药产生可能有一定的相关性。[Objective] To study the drug-resistant spectrum of MCF-7/ADM cell and the relationship of the cells to brca1 protein expression.[Methods] The drug-resistant spectrum of MCF-7/ADM cell was studied by MTT assay,and then the expression of brca1 protein was detected by immunofluoresence assay.[Results] The drug-resistant folds to ADM,VCR,and 5-FU respectively were 3.7,70.3,and 7.8.The expression of brca1 gene was high in MCF-7/ADM cells(98.23±7.63)and low in MCF-7/S cells(98.23±7.63),P<0.01.[Conclusion] MCF...

关 键 词：MCF-7/ADM细胞株 多药耐药性 BRCA1基因 

分 类 号：R737.9[医药卫生—肿瘤]