靶向DcR3的siRNA抑制人结肠癌裸鼠皮下移植瘤的实验研究  

Colon cancer subcutaneously inoculated in nude mice inhibited by siRNA to DcR3

作　　者：张胜行[1] 兰小鹏[1] 易浔飞[1] 

机构地区：[1]南京军区福州总医院检验科全军检验医学研究所,福建福州350025

出　　处：《东南国防医药》2009年第1期1-5,共5页Military Medical Journal of Southeast China

基　　金：福建省科技厅国际合作重点项目(2004I012)

摘　　要：目的以脂质体为载体,探讨靶向诱骗受体3(decoy receptor 3,DcR3)的siRNA对人结肠癌SW480细胞裸鼠皮下移植瘤生长的抑制作用。方法裸鼠背部皮下种植结肠癌SW480细胞,建立结肠癌SW480细胞裸鼠皮下移植瘤模型。针对靶标DcR3基因,利用Dharmacon公司软件设计并合成siRNA序列,通过瘤体局部多点注射脂质体与DcR3 siRNA混合物,将DcR3 siRNA转染到裸鼠背部皮下移植瘤内。同时设立阴性对照组和空白对照组。观察肿瘤治疗前后体积变化,评价DcR3 siRNA对肿瘤的抑制作用;比较肿瘤治疗前后的细胞形态学改变;免疫组织化学及RT-PCR检测DcR3基因表达;原位细胞凋亡检测肿瘤的凋亡情况。结果治疗组肿瘤体积明显小于对照组,肿瘤组织内坏死面积增大,细胞凋亡率显著增高,DcR3蛋白表达水平降低。治疗过程中裸鼠生长良好,无明显毒性反应。结论脂质体介导的DcR3 siRNA对裸鼠皮下结肠癌SW480细胞移植瘤有明显抑制、杀伤作用,细胞凋亡是DcR3 siRNA所致肿瘤细胞死亡的重要形式。Objective To investigate the effect of DcR3-siRNA mediated by liposome vector in inhibiting the growth of human cultured cancer cell SW480 subcutaneously inoculated in nude mice.Methods Athymic mice were subcutaneously transplanted with human colon carcinoma SW480 cells to establish a transplanted tumor model.To directly aim at target gene—DcR3,siRNA was synthesized by Dharmacon company and transfected into tumors subcutaneously transplanted in athymic mice.The negative and blank controls were established a...

关 键 词：结肠癌 基因治疗 SW480细胞 DCR3 siRNA 裸鼠 

分 类 号：R735.35[医药卫生—肿瘤]