兔后肢缺血诱导侧支血管生长过程中巨噬细胞浸润的变化  

CHANGES OF MACROPHAGE INVASION DURING COLLATERAL VESSEL GROWTH INDUCED BY ISCHEMIA IN RABBIT HINDLIMB

作　　者：黄庆红[1] 蔡维君[2] 吴松[3] 谢基英[4] 谢应桂[1] 

机构地区：[1]湘南学院基础医学部人体解剖学教研室,郴州423000 [2]中南大学湘雅基础医学院人体解剖学与神经生物学系,长沙410013 [3]中南大学湘雅三医院骨科,长沙410013 [4]湘南学院附属医院,郴州423000

出　　处：《中国组织化学与细胞化学杂志》2009年第1期40-43,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：湖南省教育厅科研基金资助项目(06C781;08C811)

摘　　要：目的检测兔侧支血管生长过程中各时期巨噬细胞的浸润情况;方法单侧结扎兔后肢股动脉,随后将股动脉结扎的远侧端连到相邻的静脉上造成动静脉短路,另侧为对照组,分别存活2d,1周,2周,4周后处死,用特异性的巨噬细胞抗体(RAM11)免疫荧光组织化学结合共聚焦技术检测侧支血管壁内巨噬细胞的数目及其分布;结果在正常血管,其外膜有少量巨噬细胞的存在,股动脉结扎后2d,侧支血管外膜巨噬细胞的数目增加,并黏附至内皮细胞,结扎1周后,侧支血管外膜巨噬细胞的数目显著增加,中膜有巨噬细胞的浸润,结扎2周后,侧支血管外膜巨噬细胞的数目减少,但仍高于正常血管,结扎4周后,侧支血管巨噬细胞的数目明显减少,跟正常血管情况类似,统计学分析结果表明,在结扎后2d、一周、二周和四周不同的时间,巨噬细胞在侧支血管的分布差异具有显著性;结论侧支血管发育过程中巨噬细胞的浸润和侧支血管的形成密切相关,提示巨噬细胞参与了侧支血管的生长发育。Objective To detect the change of macrophage invasion during collateral vessel growth.Methods Unilateral ligation in the rabbit hindlimb was followed by connecting the distal part of the ligated femoral artery to a nearby vein, causing artery-vein shunt. The other hindlimb served as control.The animals were killed after survival of 2d, 1week, 2weeks, and 4weeks respectively. The number and distribution of macrophages were detected by confocal immunofluorescence using the specific antibody against RAM11.Resu...

关 键 词：巨噬细胞 侧支血管生长 兔 结扎 

分 类 号：R363[医药卫生—病理学]