一种B型CpG ODN对乙肝疫苗的免疫佐剂作用  

A novel B type CpG ODN as the immune adjuvant of hepatitis B vaccine

作　　者：董博翰[1] 王莉[2] 卫红飞[1] 张培因[2] 于永利[2] 王丽颖[1] 

机构地区：[1]吉林大学白求恩医学院分子生物学教研室 [2]吉林大学白求恩医学院免疫学教研室

出　　处：《免疫学杂志》2009年第1期52-55,共4页Immunological Journal

基　　金：国家863计划项目(2006aa02z487)

摘　　要：目的研究一种本室设计的B型CpG ODN(BW006)作为乙肝疫苗佐剂的可行性。方法以琼脂糖凝胶电泳法对BW006的质量进行初步检定;H3-掺入法检测BW006对小鼠脾细胞的刺激作用;乙肝疫苗与BW006联合免疫Balb/c小鼠,并用ELISA法检测小鼠血清中的HBsAb水平。结果BW006的纯度和完整性符合实验要求,并能显著的刺激小鼠脾细胞的增生,同乙肝疫苗联用后能够明显增强乙肝疫苗的免疫效果。结论BW006对乙肝疫苗有较强的免疫佐剂作用。Objective To identify the adjuvant effects of CpG ODN(BW006) which was designed by our laboratory.Methods Agarose gel electrophoresis was used to identify the quality of BW006;the activity effect of BW006 on mouse splenocyte was detected by H3-incorporated method;Balb/c mice were immunized with hepatitis B vaccine and BW006,and then the level of HBsAb in mouse serum was assayed by ELISA.Results The purity and integrity of synthesized BW006 was qualified.This CpG ODN could significantly activate mouse spleno...

关 键 词：CPG ODN 乙型肝炎肝疫苗 佐剂 HBSAG 

分 类 号：R392.11[医药卫生—免疫学]