MPTP诱导的小鼠帕金森病模型中相关脑区iNOS时程表达变化  

The interval diversification of iNOS expression in some brain regions of MPTP models of Parkinson’s disease

作　　者：董玉林[1] 张志军[1] 刘娟[1] 郁晓燕[1] 倪衡建[1] 

机构地区：[1]南通大学医学院人体解剖学教研室神经生物学研究所江苏省神经再生重点实验室,南通226001

出　　处：《南通大学学报（医学版）》2009年第1期30-33,共4页Journal of Nantong University(Medical sciences)

基　　金：江苏省高校自然科学基金指导计划项目(06KJD310148);南通大学医学院科研基金项目(YXY2006-03)

摘　　要：目的:观察1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的小鼠急性损伤PD模型中纹状体(CPu)、黑质致密部(SNpc)、额叶皮层联合区(FrA)和腹侧被盖区(VTA)中iNOS基因和蛋白的时程表达变化。方法:用RT-PCR和Western blot方法对MPTP诱导的小鼠PD模型中相关脑区iNOS基因和蛋白的表达量进行检测。结果:MPTP诱导的小鼠PD模型中SNpc和CPu脑区iNOS基因和蛋白在给药后多个时间点均有显著性表达,其中1d是表达高峰;VTA和FrA脑区iNOS基因和蛋白的表达没有SNpc和CPu脑区表达显著。结论:表达增高的iNOS可能对SNpc和VTA脑区DA能神经元及CPu和FrA脑区的DA能神经元末梢起了损伤作用,从而导致了DA能神经元的减少及其末梢退变的发生。Objective:To observe the time-course expression of iNOS mRNA and protein in CPu,SNpc,FrA and VTA of the MPTP model of PD. Methods: Semi-quantitative RT-PCR and Western blot methods were used to detect the expressions of iNOS mRNA and proteins in these four regions. Results: The expressions of iNOS mRNA and proteins in CPu and SNpc increased significantly at several time points in the MPTP model of PD,and a peak was observed on day1.The expressions of iNOS mRNA and proteins in VTA and FrA increased less than...

关 键 词：帕金森病 1-甲基-4-苯基-1 2 3 6-四氢吡啶 诱导型一氧化氮合酶 小鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]