增强型绿色荧光蛋白在集胞藻6803中的表达  

Expression of Enhanced Green Fluorescent Protein (eGFP) in Synechocystis sp. PCC6803

作　　者：陈伟伟[1] 郭正红[1] 康升云[1] 魏兰珍[1] 王全喜[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《西北植物学报》2009年第2期229-233,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：上海市高等学校科技基金重点项目(05ZZ15);上海师范大学校基金(SK200837)

摘　　要：利用聚球藻7942热休克基因groESL的启动子和报告基因egfp,构建了表达载体pUC-Tegfp并转化集胞藻6803,并通过所制备抗体对转基因藻进行蛋白免疫印迹检测.结果发现,在转基因藻株T-egfp的细胞粗提液中含有能与eGFP抗体特异结合的蛋白质,表明外源增强型绿色荧光蛋白基因(egfp)在集胞藻6803中成功表达.The groESL gene promoter,originated from Synechococcus sp.PCC 7942,and the reporter gene egfp coding region were ligated into pUC19 vector to generate the shuttle expression plasmid,pUC-Tegfp,which was subsequently transferred into Synechocystis sp.PCC6803.Immunoblotting analysis showed the presence of eGFP protein in transgenic cells harboring pUC-Tegfp.This indicated that eGFP protein was cloned and overexpressed in transgenic cells.

关 键 词：集胞藻6803 增强型绿色荧光蛋白 转基因藻 

分 类 号：Q943.2[生物学—植物学]