匹罗卡品致痫大鼠海马神经肽Y中间神经元数目变化及其轴突出芽  

Number changes and axonal sprouting of neuropeptide Y interneurons in the hippocampus of pilocarpine-induced rats

作　　者：吴志国[1] 龙莉莉[1] 肖波[1] 陈锶[1] 易芳[1] 

机构地区：[1]中南大学湘雅医院神经内科,长沙410008

出　　处：《中南大学学报（医学版）》2009年第2期93-98,共6页Journal of Central South University ：Medical Science

基　　金：卫生部临床学科重点项目(卫规财函200 -353);湖南省科技厅一般项目(2007TP4005)~~

摘　　要：目的:探讨神经肽Y(neuropeptide Y,NPY)中间神经元在颞叶癫痫的发生和自我修复中的作用。方法:建立匹罗卡品致痫模型,应用免疫组织化学技术动态观察大鼠海马NPY中间神经元的数目变化及其轴突出芽。结果:实验组大鼠腹腔注射氯化锂-匹罗卡品后,癫痫持续状态(status epilepticus,SE)诱发成功率为92.9%,死亡率19.2%。免疫组织化学结果显示,实验组大鼠海马门区NPY中间神经元数目在SE后下降,至7d时降至最低(P<0.01),慢性期开始恢复,SE后60d时NPY神经元数目与对照组相比仍有减少(P<0.05);CA区域除SE后7dCA3区NPY神经元数目稍减少外(P<0.05),其余时间点均无明显变化(P>0.05);SE后30d齿状回分子层可见增多的NPY阳性纤维。结论:NPY中间神经元在不同部位不同时段对颞叶癫痫所致损伤的敏感性不同,NPY中间神经元的缺失在颞叶癫痫发生中起重要作用,NPY中间神经元的轴突出芽在颞叶癫痫的自我修复中起作用。Objective To investigate the role of neuropeptide Y(NPY) positive interneurons in the generation and compensation of temporal lobe epilepsy.Methods Pilocarpine-induced rat model was founded.Immunohistochemistry was used to observe the number changes and axonal sprouting of NPY interneurons at different time points in the hippocampus of rats.Results After lithium-chloride and pilocarpine administration,92.9% rats were induced status epilepticus(SE) successfully,and the mortality rate was 19.2%.In the experim...

关 键 词：神经肽Y 中间神经元 颞叶癫痫 轴突出芽 

分 类 号：R742.1[医药卫生—神经病学与精神病学]