血晶素对大鼠重症急性胰腺炎肺损伤的保护作用及其机制  

Effect of hemin on severe acute pancreatitis-associated lung injury in rats and its mechanism

作　　者：刘志勇[1] 艾宇航[1] 张丽娜[1] 

机构地区：[1]中南大学湘雅医院麻醉科,长沙410008

出　　处：《中南大学学报（医学版）》2009年第3期242-246,共5页Journal of Central South University ：Medical Science

基　　金：湖南省自然科学基金(05JJ40046)~~

摘　　要：目的:观察血晶素治疗大鼠重症急性胰腺炎(SAP)肺损伤的效果并探讨其作用机制。方法:将36只SD大鼠随机分为假手术组、SAP模型组和血晶素预处理组(n=12)。各组大鼠于制模后12h处理,光镜下观察胰腺和肺脏病理改变,应用逆转录聚合酶链反应(RT-PCR)检测大鼠肺组织诱导型血红素氧合酶(HO-1)mRNA表达情况,酶联免疫吸附试验(ELISA)法测定血清肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)水平及肺组织核因子-κB(NF-κB)活性。结果:与假手术组相比,SAP组肺间质大量中性粒细胞浸润,肺组织HO-1mRNA表达升高(P<0.05),NF-κB活性增强(P<0.05),TNF-α和IL-6水平显著升高(P<0.05)。血晶素预处理显著促进了HO-1mRNA表达,下调了NF-κB的表达活性,抑制了TNF-α和IL-6的产生,减轻了胰腺和肺组织的损伤程度,使SAP病情明显缓解。结论:血晶素具有显著的抗炎作用,明显减轻SAP时肺组织损伤,这与其促进HO-1mRNA表达,从而抑制NF-κB活性及细胞因子的产生密切相关。Objective To investigate the effect of hemin on lung injury following severe acute pancreatitis(SAP) in rats and to explore its rudimentary mechanism.Methods Thirty-six rats were randomly divided into 3 groups:a control group,a SAP model group,and a hemin-pretreated group.Rats were sacrificed 12 hours after inducing SAP model.The pathological changes of the pancreas and lungs were observed under light microscope.Expression of heme oxygenase(HO-1) mRNA was detected by reverse transcription-polymerase chain r...

关 键 词：重症急性胰腺炎 血晶素 HO-1 核因子-ΚB 

分 类 号：R576[医药卫生—消化系统]