在转基因痴呆小鼠及猕猴[^(11)C]MP4A PET显像脑乙酰胆碱酯酶活性  

[^(11)C]MP4A PET Imaging of Acetylcholinesterase in Brain of the Transgenic Mice and Rhesus Monkey

作　　者：周玲[1] 管一晖[2] 薛方平[2] 张政伟[2] 刘平[2] 丁正同[1,3] 蒋雨平[1,3] 

机构地区：[1]复旦大学医学院神经病学系 [2]复旦大学附属华山医院PET中心,200235 [3]复旦大学附属华山医院神经内科,200040

出　　处：《中国临床神经科学》2009年第1期7-11,共5页Chinese Journal of Clinical Neurosciences

基　　金：上海市经济委员会资助课题(编号:05JYYY-49)

摘　　要：目的:应用PET诊断显像剂[^(11)C]4-乙酰氧基-N-甲基哌啶([^(11)C]MP4A)对转基因痴呆小鼠、老龄小鼠及猕猴进行活体脑乙酰胆碱酯酶(AChE)活性PET显像研究。方法:小鼠经静脉注射[^(11)C]MP4A后进行PET/CT扫描,采集图像测量并计算出各感兴趣区域放射总量百分比(%ID),分析两组小鼠间及猕猴的PET显像差异与特点。扫描后即刻分离小鼠大脑、小脑和脑干,称重并测定^(11)C计数,计算放射性摄取率(%ID/g),比较两组小鼠脑放射性摄取率分布差异。结果:两组小鼠放射性显像清晰,脑放射性聚集对称。转基因痴呆鼠脑中放射性聚集较老龄鼠稀疏。转基因小鼠和老龄鼠大脑平均放射性摄取率值分别为1.49%ID/g和2.62%ID/g,差异有统计学意义(P=0.025)。猕猴脑内放射性显像清晰,放射性物质对称浓聚于皮质下,皮质聚集中等。结论:小鼠和正常猕猴[^(11)C]MP4A PET显像清晰,放射性物质对称分布于皮质下,[^(11)C]MP4A可以活体显像脑AChE的活性。Aim:To in vivo show the activity of acetylcholinesterase in the brain of transgenic mice,aged mice and adult rhesus monkey by using N-[^(11)C]methyl-4-piperidylacetate([^(11)C]MP4A) and positron emission tomography(PET).Methods:After[^(11)C]MP4A was injected through vein in the mice and monkey,those experimental animals were performed by sequential PET/CT scans.Radioactivity in regions of interest(ROI) were measured and expressed as a percentage of that in whole body(%ID).The differences of%ID in ROIs were ...

关 键 词：乙酰胆碱酯酶 正电子发射断层扫描 [^(11)C]4-乙酰氧基-N-甲基哌啶 活体显像 

分 类 号：R749.16[医药卫生—神经病学与精神病学]