RNA干扰肝癌细胞HBX基因表达及细胞增殖的实验研究  

Effect of RNA Interference on Hepatocellular Carcinoma Cell Proliferation and HBX Gene Expression

作　　者：孙会卿[1] 贺兴鄂[1] 王文龙[1] 何艳[1] 雷建华[1] 

机构地区：[1]中南大学湘雅二医院肝病研究所,长沙410011

出　　处：《中国医科大学学报》2009年第4期241-243,264,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30371402)

摘　　要：目的探讨RNA干扰技术对肝癌细胞乙型肝炎病毒X基因(HBX)表达和细胞增殖的影响。方法鉴定转染无关的对照序列(HK3细胞)和针对HBX基因shRNA的稳定肝癌细胞株(21543细胞);RT-PCR检测RNA干扰对HBX基因mRNA沉寂作用,利用流式细胞仪检测细胞周期变化。结果复苏后细胞形态差别不大;RT-PCR显示相对于MHCC-97H细胞,21543细胞的HBXmRNA水平的下降约91%,HK3细胞的HBXmRNA水平下降不明显;靶向HBX的RNA干扰后的肝癌细胞(21543细胞)较原肝癌细胞(MHCC-97H细胞)和对照细胞(HK3细胞)增殖明显减慢,而后两种细胞差别不显著;21543细胞周期显示RNA干扰后细胞延缓进入S期,增殖活性明显减低。结论RNA干扰可降低肝癌细胞HBX表达水平,并可明显抑制肝癌细胞的增殖,改变细胞生长周期。Objective To investigate the effect of HBX gene expression and growth by RNA interference on hepatocellular carcinoma cell.Methods Stable clones were established from 21543 cell lines(MHCC-97H of expressing shRNA against HBX)along with independent control MHCC-97H cell line(overexpressing HBX gene).HBX gene expression was detected after RNA interference by semi-quantitative RT-PCR and cell cycle analyzed by flow cytometry.Results There was no difference in the shape;HBX mRNA level in 21543 cell reduced 91%;...

关 键 词：肝癌细胞 RNA干扰 乙型肝炎病毒X基因 细胞周期 

分 类 号：R735.7[医药卫生—肿瘤]