非酒精性脂肪性肝病大鼠肝组织NO和iNOS的变化  

Changes of NO and iNOS Levels in Liver Tissues in Rats with Nonalcoholic Fatty Liver Disease

作　　者：吕天英[1] 卢震亚[1] 

机构地区：[1]浙江大学医学院附属第一医院,浙江杭州310003

出　　处：《浙江预防医学》2009年第4期9-11,共3页Zhejiang Journal of Preventive Medicine

基　　金：浙江省卫生厅科技项目资助(2004B041)

摘　　要：目的观察非酒精性脂肪性肝病大鼠肝组织NO和iNOS的变化,研究NO和iNOS在非酒精性脂肪性肝病中的作用机制。方法雄性SD大鼠20只,按体重随机分层分2组:正常对照组饲喂基础饲料,模型组以高脂饲料,喂养16W后处死大鼠,测体重、肝脏湿重,检测血清ALT、AST、ALP、胆碱酯酶(CHE)、总胆汁酸,病理组织学观察,采用硝酸还原法检测肝组织NO水平和以H3-精氨酸转化实验测定肝组织iNOS活性。结果16W末,模型组肝脏出现明显肝细胞脂肪变性和肝小叶炎症,肝指数、ALT、AST、ALP、胆碱酯酶(CHE)、总胆汁酸均高于对照组(P<0.05),模型组肝组织NO水平较正常组显著增高,模型组大鼠肝组织中iNOS活性也较正常组有明显提高,(P均<0.05)。结论高脂喂养脂肪肝大鼠肝脏中iNOS活性增强,以及诱导生成的NO水平也增高,iNOS,NO在脂肪肝肝损害中发挥重要作用。Objective To observe the level changes of nitric oxide(NO) and inducible nitric oxide synthase(iNOS) in rats with nonalcoholic fatty liver disease(NAFLD),and to study the role of NO and iNOS in the pathogenesis.Methods 20 male SD rats were randomly divided into 2 groups,,the control group fed with normal diet and the model group fed with high fat diet,respectively.After 16 weeks feeding,all of the rats were sacrificed to measure the body weight and wet liver weight.Besides histopathological examination,the ...

关 键 词：脂肪肝 一氧化氮 诱导型一氧化氮合成酶 

分 类 号：R575[医药卫生—消化系统]