Smad3信号通路及结缔组织生长因子在哌仑西平抑制形觉剥夺性近视中的作用机制  

Mechanism of Smad 3 signaling pathway and connective tissue growth factor in the inhibition of form deprivation myopia by pirenzepine

作　　者：籍雪颖[1] 张金嵩[1] 王艳婷[1] 孙宏亮[1] 贾沛生[1] 

机构地区：[1]郑州大学第一附属医院眼科,郑州450052

出　　处：《中南大学学报（医学版）》2009年第4期349-355,共7页Journal of Central South University ：Medical Science

摘　　要：目的:观察哌仑西平眼液对豚鼠形觉剥夺性近视的抑制效果,并探讨Smad3信号通路及结缔组织生长因子(CTGF)在哌仑西平抑制近视中可能的作用机制。方法:1周龄豚鼠40只,随机分为4组,每组10只。Ⅰ组(正常对照组):不遮盖不点药;Ⅱ组(单纯遮盖组):单纯遮盖右眼不点药;Ⅲ组(哌仑西平组):遮盖右眼,每天早晚2次点3%哌仑西平滴眼液;Ⅳ组(氮酮组):遮盖右眼,每天早晚2次点0.1%氮酮液。各组均以右眼为处理眼,左眼为对照眼。6周后检测4组豚鼠的双眼屈光度、眼轴长度,行免疫组织化学法及Western印迹法分别检测巩膜上Smad3及CTGF的蛋白表达。结果:Ⅲ组与Ⅰ组实验眼间相对性屈光度数及眼轴长度变化的差异无统计学意义(P>0.05);Ⅱ,Ⅳ组与Ⅰ组相比差异有统计学意义(P<0.05);巩膜Smad3和CTGF阳性吸光度值Ⅲ组与Ⅰ组相比差异无统计学意义(P>0.05),Ⅱ,Ⅳ组与Ⅰ组相比差异有统计学意义(P<0.05);Western印迹显示Smad3蛋白及CTGF蛋白表达Ⅱ,Ⅳ组均较Ⅰ组明显降低(P<0.05),而III组较Ⅰ组差异无统计学意义(P>0.05)。结论:哌仑西平眼液能抑制豚鼠形觉剥夺性近视的发展,推测哌仑西平可能通过影响巩膜Smad3信号通路从而抑制形觉剥夺性近视的发展。Objective To observe the inhibitive effect of pirenzepine on form deprivation myopia in guinea pigs and to explore the mechanism of Smad3 signaling pathway and connective tissue growth factor(CTGF)in the inhibition of myopia by pirenzepine.Methods Forty 1-week-old guinea pigs of either sex were randomly divided into 4 groups:a control group(Group Ⅰ),a form deprivation group(Group Ⅱ),a pirenzepine ophthalmic solution group(Group Ⅲ),and a sodium chloride ophthalmic solution group(Group Ⅳ).Translucent blinders...

关 键 词：形觉剥夺性近视 哌仑西平 SMAD3 结缔组织生长因子 豚鼠 

分 类 号：R778.11[医药卫生—眼科]