板层状鱼鳞病患者外周血白细胞生长、分化相关基因的表达  

Expressions of genes associated with growth and differentiation in peripheral blood leukocytes of lamellar ichthyosis patients

作　　者：赵蕾[1] 李红文[1] 郑乃刚[2,3] 吴景兰[2] 王一菱[2] 

机构地区：[1]郑州大学第一附属医院皮肤科,郑州450052 [2]郑州大学基础医学院分子细胞生物研究中心,郑州450001 [3]郑州大学基础医学院肿瘤基础学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2009年第2期399-402,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨板层状鱼鳞病(LI)患者外周血白细胞有关生长、分化的基因的表达情况。方法:LI患者与健康人各8例,分离外周血白细胞,采用原位杂交和RNA斑点印迹方法,检测c-fos、ras、c-myc、EGFR、iNOS基因的表达;应用免疫细胞化学染色和蛋白质斑点印迹方法,检测MAPK、Cyclin D1、Filaggrin、Involucrin及K10蛋白的表达。结果:LI组外周血白细胞c-fos、ras、c-myc、EGFR、iNOS基因和MAPK、Filaggrin、Involucrin、K10蛋白的表达较健康对照组降低(P<0.01),而Cyclin D1的表达差异无统计学意义(P>0.05)。结论:LI患者外周血白细胞有关生长、分化的基因多呈现表达下调。Aim:To explore the expressions of the genes associated with growth and differentiation in peripheral blood leukocytes of lamellar ichthyosis (LI) patients.Methods:The peripheral blood leucocytes isolated from 8 LI patients and 8 healthy volunteers were subjected to detect the expressions of MAPK,CyclinD1,Filaggerin,Involucrin and K10 by immunocytochemistry and protein dot blotting,and those of c-fos,ras,c-myc,EGFR and iNOS by in situ hybridization and RNA dot blotting.Results:The gene expressions of c-fos,r...

关 键 词：基因表达 生长 分化 白细胞 板层状鱼鳞病 

分 类 号：R758.52[医药卫生—皮肤病学与性病学]