黄芪多糖对K562细胞胎儿血红蛋白合成和细胞增殖的影响  

Influence of Astragalus Polysaccharides on Fetal Hemoglobin Synthesis and Cell Proliferation in K562 Cells

作　　者：黄为民[1] 钱新华[1] 赵丹华[1] 阳勇[1] 

机构地区：[1]南方医科大学南方医院新生儿科,广州510515

出　　处：《实用儿科临床杂志》2009年第9期687-688,695,共3页Journal of Applied Clinical Pediatrics

基　　金：广东省自然科学基金项目资助(7005146)

摘　　要：目的探讨黄芪多糖(APS)对K562细胞胎儿血红蛋白(HbF)合成和细胞增殖的影响。方法以K562细胞为模型,不同质量浓度APS(150、300、450 mg/L)诱导的细胞为实验组,丁酸钠(NaB)诱导的细胞为阳性对照,分别用Western blot和锥虫蓝拒染法计数分析HbF表达水平和对K562细胞增殖的影响。结果1.剂量效应:150、300和450 mg/L APS分别诱导K562细胞48 h,HbF合成增强(F=310.476 P=0),分别增加至未加药组的(1.56±0.03)、(1.78±0.04)和(1.51±0.32)倍,诱导前后有显著性差异。300 mg/LAPS组诱导时HbF合成最强(P=0.005)。2.时间效应:300 mg/LAPS诱导K562细胞后HbF合成逐渐增加,峰值在48～60 h,为未加药组的(2.10±0.19)倍(P=0),以后逐渐下降。与NaB组[(2.88±0.27)倍]比较,APS诱导K562细胞HbF合成有显著性差异(P=0)。3.APS对K562细胞的增殖作用:不同质量浓度(150、300和450 mg/L)APS和0.5 mmol/L NaB对K562细胞生长的影响不同(F=297.078 P=0)。APS的抑制作用随质量浓度升高而增强,但均弱于NaB(P=0.001)。APS和NaB各诱导K562细胞48 h抑制率分别为20.45%和79.55%,有显著性差异(P=0);诱导72 h抑制率分别为38.46%和90.11%,有显著性差异(P=0)。结论APS可诱导K562细胞HbF合成增加,其对K562细胞增殖的抑制作用较NaB弱。Objective To explore the effects of astragalus polysaccharides(APS) on fetal hemoglobin(HbF) synthesis and cell proli-feration in K562 cells.Methods K562 cells were chosen as the cell model and cells treated with Na-butyrate(NaB) were taken as the po-sitive control.Western blot was applied to study the level of HbF expression in K562 cells and Trypan blue dye exclusion test was employed to analyze the influence of APS(150 mg/L,300 mg/L,450 mg/L)on K562 cells proliferation.Results 1.Dosage effect:when compar...

关 键 词：Β-珠蛋白生成障碍性贫血 K562细胞 γ珠蛋白基因 黄芪多糖 

分 类 号：R285.5[医药卫生—中药学]