Plackett-Burman设计和响应面法优化荷叶总黄酮的提取工艺  

Optimization of Extraction Conditions of Total Flavonoids from Lotus Leaves by Plackett-Burman Design and Response Surface Analysis

作　　者：杨冀艳[1] 胡磊[1] 许杨[1] 

机构地区：[1]南昌大学中德联合研究院食品科学国家重点实验室,江西南昌330047

出　　处：《食品科学》2009年第6期29-33,共5页Food Science

基　　金：教育部"长江学者和创新团队发展计划"项目(IRT0540);江西省自然科学青年基金项目(2007GQN2012)

摘　　要：在单因素试验基础上,首先用Plackett-Burman设计对影响荷叶总黄酮得率的因素进行了评价,筛选出具有显著效应的三个因素——乙醇浓度、液固比和提取次数;然后用响应面分析法确定了主要影响因素的最佳提取条件为乙醇浓度67%、液固比34:1(V/m)、提取3次,在此条件下荷叶总黄酮得率的预测值可达3.19%,实测值为3.17%,两者较接近。表明Plackett-Burman设计结合响应面分析法可以很好地对荷叶总黄酮提取工艺进行优化。On the basis of single-factor tests, some factors affecting the extraction rate of total flavonoids from lotus leaves were firstly evaluated by Plackett-Burman design, and then the extraction conditions were optimized by response surface analysis. The results showed that the optimal extraction conditions are as follows: ethanol concentration 67%, ratio of ethanol solution to raw material 34∶1 and extraction times 3. Under the above conditions, the predicted value of extraction rate of total flavonoids is 3....

关 键 词：荷叶 总黄酮 PLACKETT-BURMAN设计 响应面法 

分 类 号：R284.2[医药卫生—中药学]