SD3离子交换树脂分离提取ATP的研究  

Separation of ATP from Waste Beer Yeast Hydrolysate by SD3 Ion Exchange Resin

作　　者：乔德阳[1] 甘聃[1] 

机构地区：[1]徐州工业职业技术学院化学工程系,江苏徐州221140

出　　处：《食品科学》2009年第6期128-131,共4页Food Science

摘　　要：通过对SD3离子交换树脂对ATP动态吸附、洗杂和洗脱条件的考察,确定最适吸附条件为:样品溶液pH2.0,浓度8.25g/L,离子交换柱的高径比为10(装柱湿树脂80g),流速1.0ml/min;最优洗杂液为pH1.5HC1+0.05mol/LNaCl的溶液;ATP的最优洗脱液为pH12.0NaOH+0.1mol/LNaCl溶液,优洗脱速度为0.5ml/min。The dynamic adsorption and desorption of SD3 ion exchange resin to ATP were studied. The results showed that the optimum adsorption conditions of ATP are as follows: pH of waste beer yeast hydrolysate 2.0, its concentration 1.0 mg/ml, its flow rate 8.25 g/L and diameter to height ratio of column 10 (80 g of wet resin filled into the column). The optimum eluent for removing impurity is HCl solution at pH 1.5 containing 0.05 mol/L NaCl. And the optimum elution conditions of ATP are as follows: taking NaOH sol...

关 键 词：三磷酸腺苷(ATP) 离子交换树脂 分离 

分 类 号：TQ464.3[化学工程—制药化工]